Lipidomics reveals membrane lipid remodelling and release of potential lipid mediators during early stress responses in a murine melanoma cell line

G. Balogh, Mária Péter, Gerhard Liebisch, I. Horváth, Z. Török, Enik Nagy, Andriy Maslyanko, Sándor Benk, Gerd Schmitz, John L. Harwood, L. Vígh

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

Membranes are known to respond rapidly to various environmental perturbations by changing their composition and microdomain organization. In previous work we showed that a membrane fluidizer benzyl alcohol (BA) could mimic the effects of heat stress and enhance heat shock protein synthesis in different mammalian cells. Here we explore heat- and BA-induced stress further by characterizing stress-induced membrane lipid changes in mouse melanoma B16 cells. Lipidomic fingerprints revealed that membrane stress achieved either by heat or BA resulted in pronounced and highly specific alterations in lipid metabolism. The loss in polyenes with the concomitant increase in saturated lipid species was shown to be a consequence of the activation of phopholipases (mainly phopholipase A2 and C). A phospholipase C-diacylglycerol lipase-monoacylglycerol lipase pathway was identified in B16 cells and contributed significantly to the production of several lipid mediators upon stress including the potent heat shock modulator, arachidonic acid. The accumulation of cholesterol, ceramide and saturated phosphoglyceride species with raft-forming properties observed upon both heat and BA treatments of B16 cells may explain the condensation of ordered plasma membrane domains previously detected by fluorescence microscopy and may serve as a signalling platform in stress responses or as a primary defence mechanism against the noxious effects of stresses.

Original languageEnglish
Pages (from-to)1036-1047
Number of pages12
JournalBiochimica et Biophysica Acta - Molecular and Cell Biology of Lipids
Volume1801
Issue number9
DOIs
Publication statusPublished - Sep 2010

Fingerprint

Benzyl Alcohol
Membrane Lipids
Melanoma
Hot Temperature
Lipids
Cell Line
Membranes
Monoacylglycerol Lipases
Glycerophospholipids
Polyenes
Experimental Melanomas
Lipoprotein Lipase
Ceramides
Dermatoglyphics
Type C Phospholipases
Heat-Shock Proteins
Lipid Metabolism
Fluorescence Microscopy
varespladib methyl
Arachidonic Acid

Keywords

  • Lipidomics
  • Membrane fluidity
  • Phospholipase
  • Stress signal
  • Temperature stress

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology
  • Medicine(all)

Cite this

Lipidomics reveals membrane lipid remodelling and release of potential lipid mediators during early stress responses in a murine melanoma cell line. / Balogh, G.; Péter, Mária; Liebisch, Gerhard; Horváth, I.; Török, Z.; Nagy, Enik; Maslyanko, Andriy; Benk, Sándor; Schmitz, Gerd; Harwood, John L.; Vígh, L.

In: Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids, Vol. 1801, No. 9, 09.2010, p. 1036-1047.

Research output: Contribution to journalArticle

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