Lipid rafts in the plant plasma membrane?

A. Bérczi, Gyorgy Horvath

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

In order to study the molecular characteristics of an integral membrane protein, first the protein should be solubilized. Ascorbate-reducible b-type cytochromes are highly hydrophobic integral membrane proteins with six trans-membrane α-helices. A fully ascorbate-reducible b-type cytochrome was easily solubilized by Triton X-100 from phase partition-purified plasma membranes of 5-day-old etiolated bean (Phaseolus vulgaris L.) hooks (Trost et al. 2000). However, under the very same conditions, a similar protein in the phase partition-purified plasma membrane of 9-week-old green Arabidopsis thaliana leaves seemed to be very resistant to solubilization with Triton X-100 (Bérczi et al. 2001). It was assumed that the composition of lipids in the plasma membrane of the two different tissues might influence the solubilization. Results obtained with thin layer chromatography revealed that both quantitative and qualitative differences exist between the lipid composition of the two plant plasma membranes. The observations are discussed in the light of possible existence of "lipid rafts" in the plant plasma membranes.

Original languageEnglish
Pages (from-to)7-10
Number of pages4
JournalActa Biologica Szegediensis
Volume47
Issue number1-4
Publication statusPublished - 2003

Fingerprint

Cell membranes
plasma membrane
Cell Membrane
Lipids
Cytochrome b Group
lipids
Octoxynol
cytochromes
lipid composition
solubilization
membrane proteins
Membrane Proteins
Thin layer chromatography
Phaseolus
Hooks
Thin Layer Chromatography
Chemical analysis
Arabidopsis
thin layer chromatography
Phaseolus vulgaris

Keywords

  • Lipids
  • Plant plasma membrane
  • Rafts
  • Solubilization
  • Triton X-100

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Cell Biology
  • Neuroscience(all)
  • Applied Microbiology and Biotechnology

Cite this

Lipid rafts in the plant plasma membrane? / Bérczi, A.; Horvath, Gyorgy.

In: Acta Biologica Szegediensis, Vol. 47, No. 1-4, 2003, p. 7-10.

Research output: Contribution to journalArticle

Bérczi, A & Horvath, G 2003, 'Lipid rafts in the plant plasma membrane?', Acta Biologica Szegediensis, vol. 47, no. 1-4, pp. 7-10.
Bérczi, A. ; Horvath, Gyorgy. / Lipid rafts in the plant plasma membrane?. In: Acta Biologica Szegediensis. 2003 ; Vol. 47, No. 1-4. pp. 7-10.
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