Lipid peroxidation in hemoglobin-containing liposomes. Effects of membrane phospholipid composition and cholesterol content

J. Szebeni, Klara Toth

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

The effects of phospholipid-oxidation state and vesicle composition on lipid peroxidation in hemolysate-containing liposomes (hemosomes) were studied by the thiobarbituric acid assay. Liposomes (hemosomes) were prepared from egg phosphatidylcholine (PC) with either low (PC0.08) or high (PC0.66) oxidation indices reflecting low and high conjugated diene/lipid hydroperoxy contents. Thiobarbituric acid reactivity was negligible over 6 h at 38°C in buffer-containing (control) liposomes prepared from PC0.08, whereas it was slightly increased in those prepared from PC0.66. Encapsulated hemolysate had no effect in PC0.08 liposomes, but significantly increased thiobarbituric acid reactivity in those prepared from PC0.66. Inclusion of either phosphatidylethanolamine or phosphatidylinositol in the membrane further increased lipid peroxidation in hemosomes prepared from PC0.66, whereas phosphatidic acid and phosphatidylserine were inhibitory. Inclusion of cholesterol in the membrane had no effect in PC0.66 hemosomes, but significantly inhibited lipid peroxidation in the presence of phosphatidylethanolamine or phosphatidylinositol. The effects of phosphatidic acid and cholesterol were dose-dependent. Co-incorporation of cholesterol and phosphatidic acid or phosphatidylserine in the membrane resulted in almost complete elimination of hemoglobin (Hb)-induced lipid peroxidation. Lysophosphatidic acid had similar effect as phosphatidic acid, whereas lysophosphatidylserine exerted inhibition only in the presence of phosphatidylethanolamine. The rate of lipid peroxidation showed no correlation with the amount of encapsulated Hb, neither with the oxidation indices nor the polyunsaturated fatty acid contents of negatively charged phospholipids. The above findings suggest a possible role for the high cholesterol content and preferential localization of phosphatidylserine in the inner bilayer leaflet of erythrocyte membrane in protecting against Hb-induced lipid peroxidation in the membrane.

Original languageEnglish
Pages (from-to)139-145
Number of pages7
JournalBBA - Biomembranes
Volume857
Issue number2
DOIs
Publication statusPublished - May 28 1986

Fingerprint

Liposomes
Lipid Peroxidation
Phospholipids
Hemoglobins
Cholesterol
Phosphatidic Acids
Membranes
Lipids
Chemical analysis
Phosphatidylserines
Phosphatidylinositols
Oxidation
Erythrocyte Membrane
Phosphatidylcholines
Unsaturated Fatty Acids
Ovum
Assays
Buffers
phosphatidylethanolamine
thiobarbituric acid

Keywords

  • (Liposome)
  • Cholesterol
  • Hemoglobin
  • Lipid peroxidation
  • Malondialdehyde
  • Phospholipid

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology

Cite this

Lipid peroxidation in hemoglobin-containing liposomes. Effects of membrane phospholipid composition and cholesterol content. / Szebeni, J.; Toth, Klara.

In: BBA - Biomembranes, Vol. 857, No. 2, 28.05.1986, p. 139-145.

Research output: Contribution to journalArticle

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