Lipid labeling with32P-orthophosphate and14C-acetate in marine copepods

T. Farkas, Judd C. Nevenzel, A. A. Benson

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Freshly collected Calanus pacificus were maintained in sea water containing 25 μCi/ml [32P]orthophosphate or 1 μCi/ml [14C]acetate at 10 C for 24 hr. The animals took up label from the environment and incorporated it into various lipid fractions. After incubation with [14C]acetate the order of specific activity of the different lipid classes was: phospholipids > free fatty acids > wax esters > triglycerides. Argentation thin layer chromatography of the fatty acid methyl esters showed that ca. 50% of the activity was in saturated fatty acids and 34% in polyunsaturated acids. When the animals were exposed to [32P]orthophosphate, lysophosphatidyl choline became most heavily labeled, followed by lysophosphatidyl ethanolamine, sphingomyelin, phosphatidyl ethanolamine, and phosphatidyl choline. Comparison of the data obtained with those available for decapods and mammals revealed striking similarities between these phylogenetically distant groups. It is believed that labeling the lipids of marine and freshwater planktonic crustaceans in this way will provide much information about the metabolism of lipids in these organisms.

Original languageEnglish
Pages (from-to)728-731
Number of pages4
JournalLipids
Volume8
Issue number12
DOIs
Publication statusPublished - Dec 1973

Fingerprint

Copepoda
orthophosphates
Labeling
Acetates
ethanolamine
Phosphates
acetates
choline
Lipids
Esters
Fatty Acids
lipids
Decapoda (Crustacea)
wax esters
sphingomyelins
Animals
Ethanolamine
Sphingomyelins
Calanus
Waxes

ASJC Scopus subject areas

  • Organic Chemistry
  • Cell Biology
  • Biochemistry
  • Food Science
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine (miscellaneous)

Cite this

Lipid labeling with32P-orthophosphate and14C-acetate in marine copepods. / Farkas, T.; Nevenzel, Judd C.; Benson, A. A.

In: Lipids, Vol. 8, No. 12, 12.1973, p. 728-731.

Research output: Contribution to journalArticle

Farkas, T. ; Nevenzel, Judd C. ; Benson, A. A. / Lipid labeling with32P-orthophosphate and14C-acetate in marine copepods. In: Lipids. 1973 ; Vol. 8, No. 12. pp. 728-731.
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