Lipid compositional correlates of temperature-adaptive interspecific differences in membrane physical structure

James A. Logue, Art L. De Vries, E. Fodor, Andrew R. Cossins

Research output: Contribution to journalArticle

114 Citations (Scopus)

Abstract

Teleost species from cold environments possess more disordered brain synaptic membranes than species from warm habitats, thereby providing equivalent physical structures at their respective habitat temperatures. We have related this adaptive interspecific biophysical response to the fatty acid composition of brain membranes from 17 teleost species obtained from Antarctic, temperate and semi-tropical waters, as well as from rat and turkey as representative homeotherms. Cold-adaptive increases in membrane disorder (determined by fluorescence anisotropy with diphenylhexatriene as probe) were correlated with large and linear increases in the proportion of unsaturated fatty acids, from 35 to 60% in phosphatidylcholine (PtdCho) and from 55 to 85% in phosphatidylethanolamine (PtdEth). For PtdCho, the cold-adaptive increase in unsaturation was associated almost entirely with increased proportions (from 7 to 40%) of polyunsaturated fatty acids (PUFAs), with mono-unsaturates (MUFAs) providing an approximately constant proportion in all species. Exactly opposite effects were evident for phosphatidylethanolamine (PtdEth). Thus, the compositional adaptation for PtdCho occurred largely by exchange of polyunsaturated and mono-unsaturated fatty acid in the sn-2 position, whilst for PtdEth it involved exchanges between saturates and mono-unsaturates at the sn-1 position. This difference may be related to the different molecular shapes of the two phosphoglycerides and the need to maintain the balance between bilayer-stabilising and -destabilising tendencies. This comparative study provides a more comprehensive view of the compositional adjustments that accompany and perhaps account for temperature-adaptive interspecific differences in membrane physical structure.

Original languageEnglish
Pages (from-to)2105-2115
Number of pages11
JournalJournal of Experimental Biology
Volume203
Issue number14
Publication statusPublished - Jul 2000

Fingerprint

phosphatidylethanolamines
Phosphatidylcholines
Unsaturated Fatty Acids
phosphatidylcholines
fatty acid
lipid
membrane
Lipids
Temperature
Ecosystem
Membranes
lipids
teleost
Diphenylhexatriene
Glycerophospholipids
brain
Synaptic Membranes
temperature
Fluorescence Polarization
Brain

Keywords

  • Brain
  • DPH
  • Fatty acid unsaturation
  • Fluorescence anisotropy
  • Membrane
  • Molecular species
  • Phosphatidylcholine
  • Phosphatidylethanolamine
  • Temperature adaptation

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Agricultural and Biological Sciences (miscellaneous)

Cite this

Lipid compositional correlates of temperature-adaptive interspecific differences in membrane physical structure. / Logue, James A.; De Vries, Art L.; Fodor, E.; Cossins, Andrew R.

In: Journal of Experimental Biology, Vol. 203, No. 14, 07.2000, p. 2105-2115.

Research output: Contribution to journalArticle

Logue, James A. ; De Vries, Art L. ; Fodor, E. ; Cossins, Andrew R. / Lipid compositional correlates of temperature-adaptive interspecific differences in membrane physical structure. In: Journal of Experimental Biology. 2000 ; Vol. 203, No. 14. pp. 2105-2115.
@article{6da2a36a24bb435ead555572353f74c7,
title = "Lipid compositional correlates of temperature-adaptive interspecific differences in membrane physical structure",
abstract = "Teleost species from cold environments possess more disordered brain synaptic membranes than species from warm habitats, thereby providing equivalent physical structures at their respective habitat temperatures. We have related this adaptive interspecific biophysical response to the fatty acid composition of brain membranes from 17 teleost species obtained from Antarctic, temperate and semi-tropical waters, as well as from rat and turkey as representative homeotherms. Cold-adaptive increases in membrane disorder (determined by fluorescence anisotropy with diphenylhexatriene as probe) were correlated with large and linear increases in the proportion of unsaturated fatty acids, from 35 to 60{\%} in phosphatidylcholine (PtdCho) and from 55 to 85{\%} in phosphatidylethanolamine (PtdEth). For PtdCho, the cold-adaptive increase in unsaturation was associated almost entirely with increased proportions (from 7 to 40{\%}) of polyunsaturated fatty acids (PUFAs), with mono-unsaturates (MUFAs) providing an approximately constant proportion in all species. Exactly opposite effects were evident for phosphatidylethanolamine (PtdEth). Thus, the compositional adaptation for PtdCho occurred largely by exchange of polyunsaturated and mono-unsaturated fatty acid in the sn-2 position, whilst for PtdEth it involved exchanges between saturates and mono-unsaturates at the sn-1 position. This difference may be related to the different molecular shapes of the two phosphoglycerides and the need to maintain the balance between bilayer-stabilising and -destabilising tendencies. This comparative study provides a more comprehensive view of the compositional adjustments that accompany and perhaps account for temperature-adaptive interspecific differences in membrane physical structure.",
keywords = "Brain, DPH, Fatty acid unsaturation, Fluorescence anisotropy, Membrane, Molecular species, Phosphatidylcholine, Phosphatidylethanolamine, Temperature adaptation",
author = "Logue, {James A.} and {De Vries}, {Art L.} and E. Fodor and Cossins, {Andrew R.}",
year = "2000",
month = "7",
language = "English",
volume = "203",
pages = "2105--2115",
journal = "Journal of Experimental Biology",
issn = "0022-0949",
publisher = "Company of Biologists Ltd",
number = "14",

}

TY - JOUR

T1 - Lipid compositional correlates of temperature-adaptive interspecific differences in membrane physical structure

AU - Logue, James A.

AU - De Vries, Art L.

AU - Fodor, E.

AU - Cossins, Andrew R.

PY - 2000/7

Y1 - 2000/7

N2 - Teleost species from cold environments possess more disordered brain synaptic membranes than species from warm habitats, thereby providing equivalent physical structures at their respective habitat temperatures. We have related this adaptive interspecific biophysical response to the fatty acid composition of brain membranes from 17 teleost species obtained from Antarctic, temperate and semi-tropical waters, as well as from rat and turkey as representative homeotherms. Cold-adaptive increases in membrane disorder (determined by fluorescence anisotropy with diphenylhexatriene as probe) were correlated with large and linear increases in the proportion of unsaturated fatty acids, from 35 to 60% in phosphatidylcholine (PtdCho) and from 55 to 85% in phosphatidylethanolamine (PtdEth). For PtdCho, the cold-adaptive increase in unsaturation was associated almost entirely with increased proportions (from 7 to 40%) of polyunsaturated fatty acids (PUFAs), with mono-unsaturates (MUFAs) providing an approximately constant proportion in all species. Exactly opposite effects were evident for phosphatidylethanolamine (PtdEth). Thus, the compositional adaptation for PtdCho occurred largely by exchange of polyunsaturated and mono-unsaturated fatty acid in the sn-2 position, whilst for PtdEth it involved exchanges between saturates and mono-unsaturates at the sn-1 position. This difference may be related to the different molecular shapes of the two phosphoglycerides and the need to maintain the balance between bilayer-stabilising and -destabilising tendencies. This comparative study provides a more comprehensive view of the compositional adjustments that accompany and perhaps account for temperature-adaptive interspecific differences in membrane physical structure.

AB - Teleost species from cold environments possess more disordered brain synaptic membranes than species from warm habitats, thereby providing equivalent physical structures at their respective habitat temperatures. We have related this adaptive interspecific biophysical response to the fatty acid composition of brain membranes from 17 teleost species obtained from Antarctic, temperate and semi-tropical waters, as well as from rat and turkey as representative homeotherms. Cold-adaptive increases in membrane disorder (determined by fluorescence anisotropy with diphenylhexatriene as probe) were correlated with large and linear increases in the proportion of unsaturated fatty acids, from 35 to 60% in phosphatidylcholine (PtdCho) and from 55 to 85% in phosphatidylethanolamine (PtdEth). For PtdCho, the cold-adaptive increase in unsaturation was associated almost entirely with increased proportions (from 7 to 40%) of polyunsaturated fatty acids (PUFAs), with mono-unsaturates (MUFAs) providing an approximately constant proportion in all species. Exactly opposite effects were evident for phosphatidylethanolamine (PtdEth). Thus, the compositional adaptation for PtdCho occurred largely by exchange of polyunsaturated and mono-unsaturated fatty acid in the sn-2 position, whilst for PtdEth it involved exchanges between saturates and mono-unsaturates at the sn-1 position. This difference may be related to the different molecular shapes of the two phosphoglycerides and the need to maintain the balance between bilayer-stabilising and -destabilising tendencies. This comparative study provides a more comprehensive view of the compositional adjustments that accompany and perhaps account for temperature-adaptive interspecific differences in membrane physical structure.

KW - Brain

KW - DPH

KW - Fatty acid unsaturation

KW - Fluorescence anisotropy

KW - Membrane

KW - Molecular species

KW - Phosphatidylcholine

KW - Phosphatidylethanolamine

KW - Temperature adaptation

UR - http://www.scopus.com/inward/record.url?scp=0033867362&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033867362&partnerID=8YFLogxK

M3 - Article

VL - 203

SP - 2105

EP - 2115

JO - Journal of Experimental Biology

JF - Journal of Experimental Biology

SN - 0022-0949

IS - 14

ER -