Lipase-catalyzed esterification of lactic acid in supercritical carbon dioxide

Željko Knez, Sabina Kavčič, L. Gubicza, K. Bélafi-Bakó, Gergely Németh, Mateja Primožič, Maja Habulin

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Lactate esters can be used in food industry for preservation and flavouring purpose, as well as in the pharmaceutical and cosmetic industries. Direct esterification of n-butanol and lactic acid (LA), catalyzed by immobilized lipase B from Candida antarctica (Novozyme 435), was performed in supercritical carbon dioxide (SC CO 2) with or without co-solvent. Process conditions (pressure and temperature) were optimized performing experiments in a high-pressure batch stirred-tank reactor. Experiments were carried out in the operative pressure range from 7.5 to 40 MPa and at temperatures 35°C and 55°C. The highest conversion of LA after 26 h of reaction performance was obtained in SC CO 2 with n-hexane serving as a co-solvent, at 40 MPa and 55°C. The optimal temperature and pressure for butyl lactate (BL) synthesis in SC CO 2 medium was determined at 55°C and 30 MPa. Phase behavior for LA/n-butanol/SC CO 2 system and LA/n-butanol/n-hexane/SC CO 2 system at different pressures and temperatures was also studied.

Original languageEnglish
Pages (from-to)192-197
Number of pages6
JournalJournal of Supercritical Fluids
Volume66
DOIs
Publication statusPublished - Jun 2012

Fingerprint

lactic acid
Lipases
Esterification
Lactic acid
Lipase
Carbon Dioxide
Carbon Monoxide
carbon dioxide
Lactic Acid
Carbon dioxide
1-Butanol
lactates
Butenes
Hexane
industries
temperature
Antarctic regions
Temperature
food
Cosmetics

Keywords

  • Butyl lactate
  • Candida antarctica lipase B
  • Organic solvent
  • Supercritical carbon dioxide

ASJC Scopus subject areas

  • Physical and Theoretical Chemistry
  • Chemical Engineering(all)
  • Condensed Matter Physics

Cite this

Lipase-catalyzed esterification of lactic acid in supercritical carbon dioxide. / Knez, Željko; Kavčič, Sabina; Gubicza, L.; Bélafi-Bakó, K.; Németh, Gergely; Primožič, Mateja; Habulin, Maja.

In: Journal of Supercritical Fluids, Vol. 66, 06.2012, p. 192-197.

Research output: Contribution to journalArticle

Knez, Željko ; Kavčič, Sabina ; Gubicza, L. ; Bélafi-Bakó, K. ; Németh, Gergely ; Primožič, Mateja ; Habulin, Maja. / Lipase-catalyzed esterification of lactic acid in supercritical carbon dioxide. In: Journal of Supercritical Fluids. 2012 ; Vol. 66. pp. 192-197.
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