Esterifikacija katalizovana lipazama u reakcionom sistemu sa reversnim micelama

Translated title of the contribution: Lipase-catalyzed esterification in a reversed micellar reaction system

K. Tonova, Z. Lazarova, N. Nemestóthy, L. Gubicza, K. Bélafi-Bakó

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

In this work, the lipase-catalyzed synthesis of i-amyl oleate was performed in a reversed micellar system of a cationic surfactant, CPC. The influence of the RM-system constituents on the biocatalysis characteristics (initial esterification rate and conversion extent) was studied and discussed in terms of the RM-structure. The initial water content in the RM-system (the water to surfactant mole ratio, Wo), affected both reaction parameters in a bell-shaped manner, with the maximum depending on the alcohol amount. In general the optimal Wo-values remained between 34 ÷40 for a wide range of the initial substrate ratio. The polarity (logP) and the structure of the organic solvent used as the RM-continuum strongly affected the kinetic parameters. A linear function of the initial rate on arg(logP) was established for the alkanes up to n-C10. The highest rates were measured using a medium of cycle- or branched alkanes. The esterification under inhibiting concentrations of the substrates could be intensified by a small change in the initial Wo, thus improving the enzyme RM-accommodation and manipulating the effective concentrations of both substrates around the lipase. In the studied RM-system of 0.115 mol dm-3 CPC, the maximal initial rate of the lipase-catalyzed i-amyl oleate synthesis was found to be limited at ~340 µmol min-1 g-1.

Translated title of the contributionLipase-catalyzed esterification in a reversed micellar reaction system
Original languageBosnian
Pages (from-to)175-180
Number of pages6
JournalChemical Industry and Chemical Engineering Quarterly
Volume12
Issue number3
Publication statusPublished - Jan 1 2006

Keywords

  • Cationic surfactant
  • I-amyl oleate
  • Lipase-catalyzed esterification
  • Organic solvent
  • Reversed micelles

ASJC Scopus subject areas

  • Chemical Engineering(all)

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