Lineage diversification and recombination in type-4 human astroviruses

Vito Martella, Maria C. Medici, Valentina Terio, Cristiana Catella, Giancarlo Bozzo, Fabio Tummolo, Adriana Calderaro, Floriana Bonura, Marzia Di Franco, Krisztiàn Bányai, Giovanni M. Giammanco, Simona De Grazia

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Human astroviruses (HAstVs) are important enteric pathogens and can be classified genetically and antigenically into eight types. During surveillance of HAstVs in Italy, type-4 HAstVs were detected only sporadically and found to cluster into two distinct genetic groups. Upon sequence analysis of the 3' end of the polymerase gene (ORF1b) and of the full-length ORF2, the 2008 type-4 HAstV strains were characterised as a novel ORF2 genetic lineage, designated as 4c. The 2008 type-4 HAstVs also shared the ORF1b gene with similar HAstV-4c strains detected globally, thus displaying a conserved ORF1b/ORF2 asset. By interrogation of the databases, this novel lineage 4c accounted for 60.8% of the type-4 strains identified worldwide and the vast majority of recent type-4 HAstVs. The 2002 type-4 HAstVs displayed a type-4b ORF2, whereas in the ORF1b they resembled type-1 HAstVs. This inconsistency suggests a possible recombinant origin, with the RNA switch taking place upstream the ORF1b/ORF2 junction region. Also, recombination likely played a role in the diversification of the ORF2 of the three type-4 lineages. Multi-target analysis is required for appropriate characterisation and identification of recombinant HAstVs.

Original languageEnglish
Pages (from-to)330-335
Number of pages6
JournalInfection, Genetics and Evolution
Volume20
DOIs
Publication statusPublished - Dec 2013

Keywords

  • Astrovirus
  • Epidemiology
  • Genotyping
  • Italy
  • Viral gastroenteritis

ASJC Scopus subject areas

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics
  • Molecular Biology
  • Genetics
  • Microbiology (medical)
  • Infectious Diseases

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  • Cite this

    Martella, V., Medici, M. C., Terio, V., Catella, C., Bozzo, G., Tummolo, F., Calderaro, A., Bonura, F., Di Franco, M., Bányai, K., Giammanco, G. M., & De Grazia, S. (2013). Lineage diversification and recombination in type-4 human astroviruses. Infection, Genetics and Evolution, 20, 330-335. https://doi.org/10.1016/j.meegid.2013.09.015