Differential Display RT-PCR was developed before the genomic era to serve as a tool in hunting for genes. Nowadays, applications using state-of-the-art techniques to obtain more information about the whole transcriptome or the genome have rapidly overtaken DD-RT-PCR. This paper will discuss a few of the major drawbacks and limitations of using this once highly valued method.
- Differential Display RT-PCR
- Gene expression
- Lactuca sativa L. cv. Grand Rapids
ASJC Scopus subject areas
- Agronomy and Crop Science