Limitations of using Differential Display RT-PCR in the chase for smoke-related genes

V. Soós, A. Juhász, E. Sebestyén, M. E. Light, J. Van Staden, E. Balázs

Research output: Contribution to journalArticle

Abstract

Differential Display RT-PCR was developed before the genomic era to serve as a tool in hunting for genes. Nowadays, applications using state-of-the-art techniques to obtain more information about the whole transcriptome or the genome have rapidly overtaken DD-RT-PCR. This paper will discuss a few of the major drawbacks and limitations of using this once highly valued method.

Original languageEnglish
Pages (from-to)435-441
Number of pages7
JournalActa Agronomica Hungarica
Volume56
Issue number4
DOIs
Publication statusPublished - Dec 1 2008

Keywords

  • Differential Display RT-PCR
  • Gene expression
  • Germination
  • Lactuca sativa L. cv. Grand Rapids
  • Lettuce
  • Smoke

ASJC Scopus subject areas

  • Agronomy and Crop Science

Fingerprint Dive into the research topics of 'Limitations of using Differential Display RT-PCR in the chase for smoke-related genes'. Together they form a unique fingerprint.

  • Cite this