Light-induced nuclear import of phytochrome-A: GFP fusion proteins is differentially regulated in transgenic tobacco and Arabidopsis

Lana Kim, Stefan Kircher, Reka Toth, E. Ádám, Eberhard Schäfer, Ferenc Nagy

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Abstract

Phytochromes (phy) are a family of photoreceptors that control various aspects of light-dependent plant development. Phytochrome A (phyA) is responsible for the very low fluence response (VLFR) under inductive light conditions and for the high irradiance response (HIR) under continuous far-red light. We have recently shown that nuclear import of rice phyA:GFP is regulated by VLFR in transgenic tobacco. The import is preceded by very fast, light-induced formation of sequestered areas of phyA:GFP in the cytosol. Here we report that expression of the Arabidopsis phyA-GFP fusion protein in phyA-deficient Arabidopsis plants complements the mutant phenotype. In these transgenic Arabidopsis lines, both light-dependent cytosolic formation of sequestered areas of the phyA:GFP as well as VLFR or HIR-mediated nuclear import of the fusion protein was observed. By contrast, light-dependent nuclear import of the same fusion protein was induced only by continuous far-red light (HIR) but not by pulses of far-red light (VLFR) in transgenic tobacco. These results demonstrate that photoregulation of intracellular partitioning of the Arabidopsis phyA:GFP differs significantly in different genetic backgrounds.

Original languageEnglish
Pages (from-to)125-133
Number of pages9
JournalPlant Journal
Volume22
Issue number2
DOIs
Publication statusPublished - Apr 2000

Fingerprint

Phytochrome A
Cell Nucleus Active Transport
phytochrome
Arabidopsis
imports
Tobacco
tobacco
genetically modified organisms
Light
far-red light
Proteins
proteins
Nuclear Fusion
Phytochrome
Plant Development
photoreceptors
cytosol
Cytosol
genetic background
plant development

ASJC Scopus subject areas

  • Plant Science

Cite this

Light-induced nuclear import of phytochrome-A : GFP fusion proteins is differentially regulated in transgenic tobacco and Arabidopsis. / Kim, Lana; Kircher, Stefan; Toth, Reka; Ádám, E.; Schäfer, Eberhard; Nagy, Ferenc.

In: Plant Journal, Vol. 22, No. 2, 04.2000, p. 125-133.

Research output: Contribution to journalArticle

Kim, Lana ; Kircher, Stefan ; Toth, Reka ; Ádám, E. ; Schäfer, Eberhard ; Nagy, Ferenc. / Light-induced nuclear import of phytochrome-A : GFP fusion proteins is differentially regulated in transgenic tobacco and Arabidopsis. In: Plant Journal. 2000 ; Vol. 22, No. 2. pp. 125-133.
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