Light-induced formation of 13-cis violaxanthin in leaves of Hordeum vulgare

D. Phillip, P. Molnar, G. Tóth, A. J. Young

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

The xanthophyll cycle is recognized as one of several mechanisms involved in the protection of plant membranes against damage resulting from excess excitation in high light. However, in most plants, only a proportion (typically ~ 60%) of violaxanthin undergoes de-epoxidation into zeaxanthin. When leaves of H. vulgare are illuminated at 650 μmol m-2s-1 of photosynthetically active radiation for 120 min, de-epoxidation of violaxanthin is accompanied by trans → cis isomerization of this xanthophyll. The most significant change upon illumination is the formation of the 13-cis isomer from the all-trans form of this carotenoid. The level of the 13-cis isomer increases from ~ 1% of total violaxanthin in dark-adapted leaves to ~ 9% in leaves that have been illuminated. An identical pattern of isomerization for violaxanthin is also observed in leaves which are illuminated following pre-treatment with dithiothreitol (DTT). Following illumination, plants are returned to darkness and recovery of the original isomeric content and composition is observed. The role of such light-mediated isomerization of violaxanthin in terms of limiting de-epoxidation and its potential role in photoprotection of the light-harvesting complex is discussed.

Original languageEnglish
Pages (from-to)89-95
Number of pages7
JournalJournal of Photochemistry and Photobiology, B: Biology
Volume49
Issue number2-3
DOIs
Publication statusPublished - 1999

Fingerprint

violaxanthin
Epoxidation
Hordeum
Isomerization
leaves
Hordeum vulgare
epoxidation
isomerization
Light
Isomers
Xanthophylls
Lighting
lutein
isomers
illumination
photosynthetically active radiation
lighting
darkness
carotenoids
pretreatment

Keywords

  • Cis/trans isomerization
  • Violaxanthin
  • Xanthophyll cycle

ASJC Scopus subject areas

  • Plant Science
  • Bioengineering
  • Physical and Theoretical Chemistry

Cite this

Light-induced formation of 13-cis violaxanthin in leaves of Hordeum vulgare. / Phillip, D.; Molnar, P.; Tóth, G.; Young, A. J.

In: Journal of Photochemistry and Photobiology, B: Biology, Vol. 49, No. 2-3, 1999, p. 89-95.

Research output: Contribution to journalArticle

@article{1dce158fbe724ac6ba8d0097d86ba43e,
title = "Light-induced formation of 13-cis violaxanthin in leaves of Hordeum vulgare",
abstract = "The xanthophyll cycle is recognized as one of several mechanisms involved in the protection of plant membranes against damage resulting from excess excitation in high light. However, in most plants, only a proportion (typically ~ 60{\%}) of violaxanthin undergoes de-epoxidation into zeaxanthin. When leaves of H. vulgare are illuminated at 650 μmol m-2s-1 of photosynthetically active radiation for 120 min, de-epoxidation of violaxanthin is accompanied by trans → cis isomerization of this xanthophyll. The most significant change upon illumination is the formation of the 13-cis isomer from the all-trans form of this carotenoid. The level of the 13-cis isomer increases from ~ 1{\%} of total violaxanthin in dark-adapted leaves to ~ 9{\%} in leaves that have been illuminated. An identical pattern of isomerization for violaxanthin is also observed in leaves which are illuminated following pre-treatment with dithiothreitol (DTT). Following illumination, plants are returned to darkness and recovery of the original isomeric content and composition is observed. The role of such light-mediated isomerization of violaxanthin in terms of limiting de-epoxidation and its potential role in photoprotection of the light-harvesting complex is discussed.",
keywords = "Cis/trans isomerization, Violaxanthin, Xanthophyll cycle",
author = "D. Phillip and P. Molnar and G. T{\'o}th and Young, {A. J.}",
year = "1999",
doi = "10.1016/S1011-1344(99)00019-6",
language = "English",
volume = "49",
pages = "89--95",
journal = "Journal of Photochemistry and Photobiology B: Biology",
issn = "1011-1344",
publisher = "Elsevier",
number = "2-3",

}

TY - JOUR

T1 - Light-induced formation of 13-cis violaxanthin in leaves of Hordeum vulgare

AU - Phillip, D.

AU - Molnar, P.

AU - Tóth, G.

AU - Young, A. J.

PY - 1999

Y1 - 1999

N2 - The xanthophyll cycle is recognized as one of several mechanisms involved in the protection of plant membranes against damage resulting from excess excitation in high light. However, in most plants, only a proportion (typically ~ 60%) of violaxanthin undergoes de-epoxidation into zeaxanthin. When leaves of H. vulgare are illuminated at 650 μmol m-2s-1 of photosynthetically active radiation for 120 min, de-epoxidation of violaxanthin is accompanied by trans → cis isomerization of this xanthophyll. The most significant change upon illumination is the formation of the 13-cis isomer from the all-trans form of this carotenoid. The level of the 13-cis isomer increases from ~ 1% of total violaxanthin in dark-adapted leaves to ~ 9% in leaves that have been illuminated. An identical pattern of isomerization for violaxanthin is also observed in leaves which are illuminated following pre-treatment with dithiothreitol (DTT). Following illumination, plants are returned to darkness and recovery of the original isomeric content and composition is observed. The role of such light-mediated isomerization of violaxanthin in terms of limiting de-epoxidation and its potential role in photoprotection of the light-harvesting complex is discussed.

AB - The xanthophyll cycle is recognized as one of several mechanisms involved in the protection of plant membranes against damage resulting from excess excitation in high light. However, in most plants, only a proportion (typically ~ 60%) of violaxanthin undergoes de-epoxidation into zeaxanthin. When leaves of H. vulgare are illuminated at 650 μmol m-2s-1 of photosynthetically active radiation for 120 min, de-epoxidation of violaxanthin is accompanied by trans → cis isomerization of this xanthophyll. The most significant change upon illumination is the formation of the 13-cis isomer from the all-trans form of this carotenoid. The level of the 13-cis isomer increases from ~ 1% of total violaxanthin in dark-adapted leaves to ~ 9% in leaves that have been illuminated. An identical pattern of isomerization for violaxanthin is also observed in leaves which are illuminated following pre-treatment with dithiothreitol (DTT). Following illumination, plants are returned to darkness and recovery of the original isomeric content and composition is observed. The role of such light-mediated isomerization of violaxanthin in terms of limiting de-epoxidation and its potential role in photoprotection of the light-harvesting complex is discussed.

KW - Cis/trans isomerization

KW - Violaxanthin

KW - Xanthophyll cycle

UR - http://www.scopus.com/inward/record.url?scp=0032993865&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032993865&partnerID=8YFLogxK

U2 - 10.1016/S1011-1344(99)00019-6

DO - 10.1016/S1011-1344(99)00019-6

M3 - Article

AN - SCOPUS:0032993865

VL - 49

SP - 89

EP - 95

JO - Journal of Photochemistry and Photobiology B: Biology

JF - Journal of Photochemistry and Photobiology B: Biology

SN - 1011-1344

IS - 2-3

ER -