Light-induced changes in glutamate release from isolated rat retina is regulated by cyclic guanosine monophosphate

Péter Barabás, Ilona Kovács, Richárd Kovács, János Pálhalmi, Julianna Kardos, Arne Schousboe

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Isolated rat retina was preloaded with [14C]glutamate and subsequently superfused to follow release of glutamate (Glu). After 20 min of superfusion in the dark, exposure of the [14C]Glu preloaded rat retina to a single train of white light pulses reduced Glu efflux significantly in the absence as well as in the presence of low (4 μM) and high (0.5 mM) concentrations of the Glu uptake inhibitor trans-L-pyrrolidine-2,4-dicarboxylate (t-PDC). The dark-light response was the highest in the presence of 4 μM t-PDC by establishing a plateau at 75% ± 7% of the tonic Glu release in the dark (100%). Displaying transient to saturating responses with increasing relative luminance, time series of four trains of white light pulses arrived at a plateau of 85% ± 10%. The cyclic guanosine monophosphate (cGMP) phosphodiesterase inhibitor Zaprinast (200 μM) antagonized the effect of the light series, leading to a plateau of 115% ± 9%. Exposure of the retina to the guanylyl cyclase inhibitor LY83583 (30 and 100 μM) showed fast, transient responses characterized by peaks at 90% ± 1% and 80% ± 3%, respectively.

Original languageEnglish
Pages (from-to)149-155
Number of pages7
JournalJournal of Neuroscience Research
Volume67
Issue number2
DOIs
Publication statusPublished - Jan 15 2002

Fingerprint

Cyclic GMP
Retina
Glutamic Acid
Light
6-anilino-5,8-quinolinedione
Phosphodiesterase Inhibitors
Guanylate Cyclase

Keywords

  • [C]Glu efflux
  • Photoresponse
  • Rat retina
  • Trans-L-pyrrolidine-2,4-dicarboxylate LY83583
  • Zaprinast

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Light-induced changes in glutamate release from isolated rat retina is regulated by cyclic guanosine monophosphate. / Barabás, Péter; Kovács, Ilona; Kovács, Richárd; Pálhalmi, János; Kardos, Julianna; Schousboe, Arne.

In: Journal of Neuroscience Research, Vol. 67, No. 2, 15.01.2002, p. 149-155.

Research output: Contribution to journalArticle

Barabás, Péter ; Kovács, Ilona ; Kovács, Richárd ; Pálhalmi, János ; Kardos, Julianna ; Schousboe, Arne. / Light-induced changes in glutamate release from isolated rat retina is regulated by cyclic guanosine monophosphate. In: Journal of Neuroscience Research. 2002 ; Vol. 67, No. 2. pp. 149-155.
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