Light- and electron-microscopic studies on the formation of "dark" variants of chromaffin cells in the rat adrenal medulla

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Abstract

"Dark" cell formation has been studies in the adrenal medulla with combined light- and electron-microscopic methods. Correlation between light- and electron-microscopic appearance has been demonstrated. It has been pointed out that the formation of "dark" cell artefact can be prevented during immersion fixation and can be produced also during perfusion fixation by appropriate physical conditions. "Dark" cell formation was elicited by prefixation cooling and/or by increased tissular pressure. The nature of the underlying physicochemical change was interpreted as a uniformly distributed sol-gel transition of cytoplasmic macromolecules. The formation of cytoplasmic gel structures can be considered an endothermic process which involves volume increase (ΔV)-as may be inferred by their susceptibility to solation by high pressure and low temperature.

Original languageEnglish
Pages (from-to)61-69
Number of pages9
JournalHistochemistry
Volume48
Issue number1
DOIs
Publication statusPublished - Mar 1976

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Chromaffin Cells
Adrenal Medulla
Electrons
Light
Gels
Cytoplasmic Structures
Pressure
Immersion
Polymethyl Methacrylate
Artifacts
Perfusion
Temperature

ASJC Scopus subject areas

  • Anatomy

Cite this

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