Light- and electron-microscopic demonstration of immunoreactive opsin in the pinealocytes of various vertebrates

B. Vígh, I. Vigh-Teichmann

Research output: Contribution to journalArticle

86 Citations (Scopus)

Abstract

An antibody to opsin isolated from rod outer segments of the frog retina was applied in light- and electron-microscopic immunocytochemical studies to the pineal organ of various vertebrates (Cyprinus carpio, Carassius auratus, Rana esculenta, Emys orbicularis, Pseudemys scripta elegans, Lacerta agilis and viridis, Gallus domesticus, Columba livia, Melopsittacus undulatus, Serinus canaria, Taeniopyga punctata, Didelphis virginiana, Erinaceus roumanicus, Myotis myotis, rabbit, rat, cat). A strong immunoreaction was visible in the outer segments of the pinealocytes of one anuran and several teleost species. The outer segments of pinealocytes in the chelonian reptiles and birds also contained immunoreactive opsin. Ultrastructurally, PAP complexes were localized to the photoreceptor membranes of the outer segments. Immunoreactivity for opsin could not be demonstrated in the lacertilian parietal eye and pineal organ. In the opossum (Marsupialia), pinealocytes remain in contact with the intrapineal invaginations of the pineal recess. In insectivores, the cilia of the pinealocytes exhibit a relation to glial cells similar to that between the outer segments of retinal photoreceptors and the pigment cells. The cilia of mammalian pinealocytes did not show a clear-cut immunoreactivity to opsin with the electron-microscopic technique employed.

Original languageEnglish
Pages (from-to)451-463
Number of pages13
JournalCell and Tissue Research
Volume221
Issue number2
DOIs
Publication statusPublished - Dec 1981

Fingerprint

Opsins
Vertebrates
Demonstrations
Electrons
Light
Cilia
Melopsittacus
Didelphis
Canaries
Rana esculenta
Rod Cell Outer Segment
Opossums
Marsupialia
Vertebrate Photoreceptor Cells
Goldfish
Reptiles
Retinal Pigments
Carps
Columbidae
Birds

Keywords

  • Comparative aspects (vertebrates)
  • Opsin immunoreactivity
  • Pineal photoreceptors
  • Pinealocytes
  • Rhodopsin-like photopigments

ASJC Scopus subject areas

  • Anatomy
  • Clinical Biochemistry
  • Cell Biology

Cite this

Light- and electron-microscopic demonstration of immunoreactive opsin in the pinealocytes of various vertebrates. / Vígh, B.; Vigh-Teichmann, I.

In: Cell and Tissue Research, Vol. 221, No. 2, 12.1981, p. 451-463.

Research output: Contribution to journalArticle

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