Light- and dark-adaptation of bacteriorhodopsin measured by a photoelectric method

György Váró, Krzysztof Bryl

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

The process of light- and dark-adaptation of bacteriorhodopsin was studied by measuring the photoelectric response signal corresponding to the M form of the light-adapted photocycle. Two different kinds of sample were used: oriented dried samples and oriented gel samples. It was shown that the quantum efficiency of light-adaptation does not depend on the temperature of the sample, but strongly depends upon the water content, which means that in this process the determining step of the branching is the light excitation (bacteriorhodopsin to bacteriorhodopsin * or bacteriorhodopsin * to the next state), not a later step during the 13-cis photocycle. The process of dark-adaptation depends upon both the temperature and the humidity of the sample.

Original languageEnglish
Pages (from-to)247-252
Number of pages6
JournalBBA - Bioenergetics
Volume934
Issue number2
DOIs
Publication statusPublished - Jul 6 1988

    Fingerprint

Keywords

  • Bacteriorhodopsin
  • Dark-adaptation
  • Light-adaptation
  • Oriented film
  • Photocycle
  • Photoelectric signal

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Cell Biology

Cite this