Light-adaptation attenuates the effects of phosphodiesterase blockade by Zaprinast in the isolated rat retina

Péter Barabás, Károly Antal, Julianna Kardos

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The effect of the type V/VI-selective phosphodiesterase inhibitor, Zaprinast, (200 μM) on the light-evoked extracellular field potential (EFP) in the isolated rat retina was tested under dark- and light-adapted conditions at two different temperatures. Peak enhancement EFP in dark- (344±70%; mean±SEM) and light-adapted (182±31%) retina at 37°C was reached within 3 min of treatment with Zaprinast (200 μM) followed by a slower decrease to a level of 85±14 and 26±7% in dark- and light-adapted retina, respectively. The effect of Zaprinast (20 μM) on the pharmacologically-isolated photoreceptor component of the EFP was lost with increasing levels of background light. This may suggest that there is a slow time scale (minutes) shift in the steady state level of cGMP during light-adaptation.

Original languageEnglish
Pages (from-to)195-198
Number of pages4
JournalNeuroscience Letters
Volume357
Issue number3
DOIs
Publication statusPublished - Mar 11 2004

Keywords

  • Adaptation
  • Cyclic guanosine monophosphate
  • Electric light response
  • Isolated rat retina
  • Phosphodiesterase
  • Zaprinast

ASJC Scopus subject areas

  • Neuroscience(all)

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