Ligands of purinergic receptors stimulate electrogenic H+-transport of neutrophils

Katalin Suszták, Krisztina Káldi, András Kapus, Erzsébet Ligeti

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The possible role of ATP, acting as a ligand on cell surface receptor was investigated in the activation of the electrogenic H+-transporting pathway of porcine neutrophil granulocytes. (1) ATP brought about 2.1-fold increase in the rate of H+-efflux. (2) The order of potency of different nucleotides suggests, that ATP acts on P2 type purinoceptor. (3) The effect of the nucleotides was prevented by inhibition of phospholipase A2. (4) Inhibition of the metabolism of arachidonic acid (AA) via the cyclooxygenase pathway had no effect, whereas inhibition of the lipoxygenase pathway significantly enhanced H+-release. This is the first report about activation of the H+-transporter by physiological stimulator acting on the cell surface.

Original languageEnglish
Pages (from-to)79-82
Number of pages4
JournalFEBS letters
Volume375
Issue number1-2
DOIs
Publication statusPublished - Nov 13 1995

Keywords

  • Adenosine triphosphate
  • Arachidonic acid
  • Electrogenic H-transporter
  • Neutrophil granulocyte
  • Phopholipase A

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Fingerprint Dive into the research topics of 'Ligands of purinergic receptors stimulate electrogenic H<sup>+</sup>-transport of neutrophils'. Together they form a unique fingerprint.

  • Cite this