Life on a microarray: Assessing live cell functions in a microarray format

Krisztián Papp, Zoltán Szittner, József Prechl

Research output: Contribution to journalReview article

20 Citations (Scopus)

Abstract

Microarray technology outgrew the detection of simple intermolecular interactions, as incubation of slides with living cells opened new vistas. Cell-based array technology permits simultaneous detection of several different cell surface molecules, allowing the complex characterization of cells with an amount of information that is hardly assessed by any other technique. Furthermore, binding of cells to printed antibodies or ligands may induce their activation, and consequently the outcome of these interactions, such as phosphorylation, gene expression, secretion of various products; differentiation, proliferation and apoptosis of the cells are also measurable on arrays. Moreover, since cells can be transfected with printed vectors, over- or under-expression of selected genes is also achievable simultaneously, creating a nice tool for assessing the function of a given gene. The enormously highthroughput cell-based microarray technology enables testing the effect of external stimuli on a scale that was earlier unthinkable. This review summarizes the possible applications of cell-based arrays.

Original languageEnglish
Pages (from-to)2717-2725
Number of pages9
JournalCellular and Molecular Life Sciences
Volume69
Issue number16
DOIs
Publication statusPublished - Aug 1 2012

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Keywords

  • Cell activation
  • Cell binding
  • Functional test
  • Microarray

ASJC Scopus subject areas

  • Molecular Medicine
  • Molecular Biology
  • Pharmacology
  • Cellular and Molecular Neuroscience
  • Cell Biology

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