Levels of total antioxidant capacity and 8-hydroxy-2′-deoxyguanosine of serum and follicular fluid in women undergoing in vitro fertilization: focusing on endometriosis

Ákos Várnagy, T. Kőszegi, Erzséber Györgyi, Sarolta Szegedi, E. Sulyok, Viktória Prémusz, József Bódis

Research output: Contribution to journalArticle

Abstract

This study was designed to evaluate the levels of oxidative stress (OS) markers, total non-enzymatic antioxidant capacity (TAC) and 8-hydroxy-2′-deoxyguanosine (8-OHdG) in the serum and follicular fluid (FF) of patients undergoing in vitro fertilization (IVF). The impact of these biomarkers on IVF outcome was also analysed. Samples were obtained from 61 patients (age: 36.40 ± 4.99 years; BMI: 22.54 ± 2.46; infertility: 3.7 ± 2.5 years [mean ± SD]) before and after controlled ovarian hyperstimulation (COH). Patients with and without endometriosis were also evaluated separately. TAC was quantified by enhanced chemiluminescence assay and 8-OHdG was measured by enzyme-linked immunosorbent assay. It was demonstrated that these biomarkers responded to COH differently. No relationship could be detected in their FF levels, although their cumulative serum levels were inversely related. Both FF TAC and FF 8-OHdG had a negative impact on the number of good quality embryos, but an effect of Serum TAC and 8-OHdG could not be observed. When women with and without endometriosis were evaluated separately, inconsistent results were obtained. However, women without endometriosis had higher levels of serum and FF TAC when they progressed to clinical pregnancy. Our findings support the notion that OS has an important contribution to the reproductive potential in IVF patients, the ideal biomarkers of outcome measures, however, need to be further explored.

Original languageEnglish
JournalHuman Fertility
DOIs
Publication statusAccepted/In press - Jan 1 2018

Fingerprint

Follicular Fluid
Endometriosis
Fertilization in Vitro
Antioxidants
Serum
Biomarkers
Oxidative Stress
Luminescence
Infertility
Embryonic Structures
Enzyme-Linked Immunosorbent Assay
Outcome Assessment (Health Care)
8-oxo-7-hydrodeoxyguanosine
Pregnancy

Keywords

  • embryo quality
  • endometriosis
  • good quality oocytes
  • Oxidative stress
  • reproductive potential

ASJC Scopus subject areas

  • Reproductive Medicine
  • Obstetrics and Gynaecology

Cite this

Levels of total antioxidant capacity and 8-hydroxy-2′-deoxyguanosine of serum and follicular fluid in women undergoing in vitro fertilization : focusing on endometriosis. / Várnagy, Ákos; Kőszegi, T.; Györgyi, Erzséber; Szegedi, Sarolta; Sulyok, E.; Prémusz, Viktória; Bódis, József.

In: Human Fertility, 01.01.2018.

Research output: Contribution to journalArticle

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