99mTc-HMPAO was prepared by incubating for different times (range of 7-90 min) and was then used for in vitro leukocyte labeling. The effects of the variation of incubation time and cell concentration in the labeling suspension on the yield of the lipophilic complex, the labeling efficacy and the image quality were determined. The yield of lipophilic complex decreased with an increase of incubation time (r = -0.5335). A weak correlation was observed between the incubation time and the labeling efficacy (r = -0.2275) and between the lipophilic complex yield and the labeling efficacy (r = -0.1960). The cell concentration in the labeling suspension appeared to be the most important factor affecting the labeling efficacy (r = -0.4996). Variation of incubation time did not exert a change in quality of the images. The 99mTc-HMPAO kit containing a stabilizing agent can be used for multiple leukocyte labeling after incubation for 7-90 min.
ASJC Scopus subject areas
- Molecular Medicine
- Radiology Nuclear Medicine and imaging
- Cancer Research