Letter: Analysis of complex oligosaccharides using graphitized carbon liquid chromatography/mass spectrometry

Lívia Budai, Ferenc Pollreisz, Olivér Ozohanics, K. Ludányi, L. Drahos, K. Vékey

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The sugar fraction of α-1 acid glycoprotein (AGP) was studied using porous graphitized carbon (PGC) chromatography coupled to mass spectrometry. Electrospray ionization provides efficient control over fragmentation; at low collision energy only molecular species were observed, allowing accurate oligosaccharide profiling. PGC chromatography was useful separating 18 sugars differing in monosaccharide composition. Most of these were separated into several isomeric forms; altogether 49 different oligosaccharides were found in AGP.

Original languageEnglish
Pages (from-to)419-422
Number of pages4
JournalEuropean Journal of Mass Spectrometry
Volume14
Issue number6
DOIs
Publication statusPublished - 2008

Fingerprint

Liquid chromatography
liquid chromatography
chromatography
sugars
Chromatography
Oligosaccharides
Sugars
Mass spectrometry
Glycoproteins
mass spectroscopy
Carbon
monosaccharides
Electrospray ionization
acids
Acids
Monosaccharides
carbon
fragmentation
ionization
collisions

Keywords

  • Glycoform
  • Glycoprotein
  • Graphitized carbon chromatography (GPC)
  • HPLC
  • Isoform
  • Mass spectrometry
  • Oligosaccharide
  • UPLC

ASJC Scopus subject areas

  • Spectroscopy
  • Atomic and Molecular Physics, and Optics

Cite this

Letter : Analysis of complex oligosaccharides using graphitized carbon liquid chromatography/mass spectrometry. / Budai, Lívia; Pollreisz, Ferenc; Ozohanics, Olivér; Ludányi, K.; Drahos, L.; Vékey, K.

In: European Journal of Mass Spectrometry, Vol. 14, No. 6, 2008, p. 419-422.

Research output: Contribution to journalArticle

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