Leptin and leptin binding activity in the preovulatory follicle of polycystic ovary syndrome patients.

P. Fedorcsák, R. Storeng, P. O. Dale, T. Tanbo, P. Torjesen, J. Urbancsek, T. Abyholm

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

To investigate the clinical importance of leptin's intraovarian effects, we studied the concentration of leptin and leptin binding activity in the plasma and in the follicular fluid of PCOS patients (n=20; median BMI: 27.1 kg/m2, range 19.7-36.3) undergoing controlled ovarian stimulation with long-term GnRH agonist, recombinant FSH, and in vitro fertilization. Follicular fluid and blood samples were collected during follicle aspiration for IVF. Total leptin concentration was measured by radioimmunoassay, and specific leptin binding activity was accessed by a gel filtration column assay. Follicular fluid and plasma leptin levels were similar (median 1135 pmol/l vs. 1409 pmol/l; p=0.81). Follicular fluid to plasma leptin ratio was independently associated with cumulative FSH dose (r=0.63; p=0.006) and insulin resistance index (r=-0.45; p=0.04). Specific leptin binding activity was higher in the plasma than in the follicular fluid [median 7.94% vs. 3.49%; p

Original languageEnglish
Pages (from-to)649-655
Number of pages7
JournalScandinavian Journal of Clinical and Laboratory Investigation
Volume60
Issue number8
Publication statusPublished - Dec 2000

Fingerprint

Polycystic Ovary Syndrome
Leptin
Follicular Fluid
Fluids
Plasmas
Ovulation Induction
Fertilization in Vitro
Gonadotropin-Releasing Hormone
Radioimmunoassay
Gel Chromatography
Insulin Resistance
Assays
Blood
Gels
Insulin

ASJC Scopus subject areas

  • Clinical Biochemistry

Cite this

Leptin and leptin binding activity in the preovulatory follicle of polycystic ovary syndrome patients. / Fedorcsák, P.; Storeng, R.; Dale, P. O.; Tanbo, T.; Torjesen, P.; Urbancsek, J.; Abyholm, T.

In: Scandinavian Journal of Clinical and Laboratory Investigation, Vol. 60, No. 8, 12.2000, p. 649-655.

Research output: Contribution to journalArticle

Fedorcsák, P. ; Storeng, R. ; Dale, P. O. ; Tanbo, T. ; Torjesen, P. ; Urbancsek, J. ; Abyholm, T. / Leptin and leptin binding activity in the preovulatory follicle of polycystic ovary syndrome patients. In: Scandinavian Journal of Clinical and Laboratory Investigation. 2000 ; Vol. 60, No. 8. pp. 649-655.
@article{131bec5a5b9340deafed493d69a991f9,
title = "Leptin and leptin binding activity in the preovulatory follicle of polycystic ovary syndrome patients.",
abstract = "To investigate the clinical importance of leptin's intraovarian effects, we studied the concentration of leptin and leptin binding activity in the plasma and in the follicular fluid of PCOS patients (n=20; median BMI: 27.1 kg/m2, range 19.7-36.3) undergoing controlled ovarian stimulation with long-term GnRH agonist, recombinant FSH, and in vitro fertilization. Follicular fluid and blood samples were collected during follicle aspiration for IVF. Total leptin concentration was measured by radioimmunoassay, and specific leptin binding activity was accessed by a gel filtration column assay. Follicular fluid and plasma leptin levels were similar (median 1135 pmol/l vs. 1409 pmol/l; p=0.81). Follicular fluid to plasma leptin ratio was independently associated with cumulative FSH dose (r=0.63; p=0.006) and insulin resistance index (r=-0.45; p=0.04). Specific leptin binding activity was higher in the plasma than in the follicular fluid [median 7.94{\%} vs. 3.49{\%}; p",
author = "P. Fedorcs{\'a}k and R. Storeng and Dale, {P. O.} and T. Tanbo and P. Torjesen and J. Urbancsek and T. Abyholm",
year = "2000",
month = "12",
language = "English",
volume = "60",
pages = "649--655",
journal = "Scandinavian Journal of Clinical and Laboratory Investigation",
issn = "0036-5513",
publisher = "Informa Healthcare",
number = "8",

}

TY - JOUR

T1 - Leptin and leptin binding activity in the preovulatory follicle of polycystic ovary syndrome patients.

AU - Fedorcsák, P.

AU - Storeng, R.

AU - Dale, P. O.

AU - Tanbo, T.

AU - Torjesen, P.

AU - Urbancsek, J.

AU - Abyholm, T.

PY - 2000/12

Y1 - 2000/12

N2 - To investigate the clinical importance of leptin's intraovarian effects, we studied the concentration of leptin and leptin binding activity in the plasma and in the follicular fluid of PCOS patients (n=20; median BMI: 27.1 kg/m2, range 19.7-36.3) undergoing controlled ovarian stimulation with long-term GnRH agonist, recombinant FSH, and in vitro fertilization. Follicular fluid and blood samples were collected during follicle aspiration for IVF. Total leptin concentration was measured by radioimmunoassay, and specific leptin binding activity was accessed by a gel filtration column assay. Follicular fluid and plasma leptin levels were similar (median 1135 pmol/l vs. 1409 pmol/l; p=0.81). Follicular fluid to plasma leptin ratio was independently associated with cumulative FSH dose (r=0.63; p=0.006) and insulin resistance index (r=-0.45; p=0.04). Specific leptin binding activity was higher in the plasma than in the follicular fluid [median 7.94% vs. 3.49%; p

AB - To investigate the clinical importance of leptin's intraovarian effects, we studied the concentration of leptin and leptin binding activity in the plasma and in the follicular fluid of PCOS patients (n=20; median BMI: 27.1 kg/m2, range 19.7-36.3) undergoing controlled ovarian stimulation with long-term GnRH agonist, recombinant FSH, and in vitro fertilization. Follicular fluid and blood samples were collected during follicle aspiration for IVF. Total leptin concentration was measured by radioimmunoassay, and specific leptin binding activity was accessed by a gel filtration column assay. Follicular fluid and plasma leptin levels were similar (median 1135 pmol/l vs. 1409 pmol/l; p=0.81). Follicular fluid to plasma leptin ratio was independently associated with cumulative FSH dose (r=0.63; p=0.006) and insulin resistance index (r=-0.45; p=0.04). Specific leptin binding activity was higher in the plasma than in the follicular fluid [median 7.94% vs. 3.49%; p

UR - http://www.scopus.com/inward/record.url?scp=0034573645&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034573645&partnerID=8YFLogxK

M3 - Article

C2 - 11218147

AN - SCOPUS:0034573645

VL - 60

SP - 649

EP - 655

JO - Scandinavian Journal of Clinical and Laboratory Investigation

JF - Scandinavian Journal of Clinical and Laboratory Investigation

SN - 0036-5513

IS - 8

ER -