Length of the polyadenylated sequence in cytoplasmic ribonucleic acid isolated from fetal calf myoblasts differentiating in vitro

S. Goto, Margaret Buckingham, François Gros

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2 Citations (Scopus)

Abstract

Poly(adenylic acid) [poly(A)] containing cytoplasmic ribonucleic acid (RNA) in differentiating fetal calf myoblasts cultivated in vitro was examined by hybridization with radioactive poly(uridylic acid). The size distribution of the poly(A)-containing RNA after sucrose-gradient centrifugation was similar in cells before and after differentiation. There was no apparent correlation between the length of the poly(A) segment and the change in stability of messenger RNA which occurs on differentiation, nor with the polysomal or nonpolysomal localization of the RNA in the cytoplasm. The average length of the poly(A) segments in cytoplasmic RNA in the steady state was found to be dependent on the size of the RNA: the longer the RNA, the longer the average length of the poly(A) sequence. In contrast, in pulse-labeled RNA, the length of poly(A) is similar in all size classes of RNA.

Original languageEnglish
Pages (from-to)5449-5457
Number of pages9
JournalBiochemistry
Volume20
Issue number19
Publication statusPublished - 1981

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Myoblasts
RNA
Adenosine Monophosphate
Uridine Monophosphate
In Vitro Techniques
Centrifugation
Sucrose
Cytoplasm

ASJC Scopus subject areas

  • Biochemistry

Cite this

Length of the polyadenylated sequence in cytoplasmic ribonucleic acid isolated from fetal calf myoblasts differentiating in vitro. / Goto, S.; Buckingham, Margaret; Gros, François.

In: Biochemistry, Vol. 20, No. 19, 1981, p. 5449-5457.

Research output: Contribution to journalArticle

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