Lectin-induced increase in clonogenic growth of haematopoietic progenitor cells

Katalin Vehmeyer, T. Hajtó, Katarina Hostanska, Stefan Könemann, Holger Löser, Reinhard Saller, Bernhard Wörmann

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

The galactoside-specific plant lectin, Viscum album agglutinin (VAA-I) increases cellular parameters of natural host defence. It also binds to a variety of haematopoietic cells, including progenitors. We investigated whether VAA-I has a stimulatory effect on haematopoietic progenitor cells. Peripheral blood progenitor cells from 7 healthy volunteers were cultured in a colony assay with VAA-I plus erythropoietin (EPO) and stem cell factor (SCF). At 50 pg/ml VAA-I induced a significant increase in the cytokine- dependent clonogenic growth (52% in median, p <0.05). In another set of experiments purified CD34+ cells were isolated from the bone marrow aspirate of 4 patients with non-metastatic breast cancer using fluorescence-activated cell sorting. Binding to CD34+ cells was demonstrated by using directly fluorescence-conjugated VAA-I. Co-incubation with D-galactose significantly abrogated this effect. CD34+ cells were cultured in the presence of EPO, SCF, interleukin-3, granulocyte/monocyte colony-stimulating factor and granulocyte colony-stimulating factor. VAA-I alone had no measurable effect on the clonogenic growth of the isolated cells. However, at concentrations of 100 and 250 pg/ml VAA-I increased the cytokine-dependent proliferation and differentiation of CD34+ cells by a median of 75 and 85%, respectively. The results show that VAA-I binds to haematopoietic progenitor cells and has a co-stimulatory effect on their proliferation.

Original languageEnglish
Pages (from-to)16-20
Number of pages5
JournalEuropean Journal of Haematology
Volume60
Issue number1
Publication statusPublished - 1998

Fingerprint

Viscum album
Agglutinins
Hematopoietic Stem Cells
Lectins
Growth
Stem Cell Factor
Granulocyte Colony-Stimulating Factor
Erythropoietin
Plant Lectins
Cytokines
Galactosides
Interleukin-3
Galactose
Monocytes
Cell Differentiation
Cultured Cells
Blood Cells
Healthy Volunteers
Flow Cytometry
Stem Cells

Keywords

  • CD34 cells
  • Colony-forming assay
  • Viscum album agglutinin

ASJC Scopus subject areas

  • Hematology

Cite this

Vehmeyer, K., Hajtó, T., Hostanska, K., Könemann, S., Löser, H., Saller, R., & Wörmann, B. (1998). Lectin-induced increase in clonogenic growth of haematopoietic progenitor cells. European Journal of Haematology, 60(1), 16-20.

Lectin-induced increase in clonogenic growth of haematopoietic progenitor cells. / Vehmeyer, Katalin; Hajtó, T.; Hostanska, Katarina; Könemann, Stefan; Löser, Holger; Saller, Reinhard; Wörmann, Bernhard.

In: European Journal of Haematology, Vol. 60, No. 1, 1998, p. 16-20.

Research output: Contribution to journalArticle

Vehmeyer, K, Hajtó, T, Hostanska, K, Könemann, S, Löser, H, Saller, R & Wörmann, B 1998, 'Lectin-induced increase in clonogenic growth of haematopoietic progenitor cells', European Journal of Haematology, vol. 60, no. 1, pp. 16-20.
Vehmeyer K, Hajtó T, Hostanska K, Könemann S, Löser H, Saller R et al. Lectin-induced increase in clonogenic growth of haematopoietic progenitor cells. European Journal of Haematology. 1998;60(1):16-20.
Vehmeyer, Katalin ; Hajtó, T. ; Hostanska, Katarina ; Könemann, Stefan ; Löser, Holger ; Saller, Reinhard ; Wörmann, Bernhard. / Lectin-induced increase in clonogenic growth of haematopoietic progenitor cells. In: European Journal of Haematology. 1998 ; Vol. 60, No. 1. pp. 16-20.
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