Lectin-immobilization strategies for affinity purification and separation of glycoconjugates

Alex Monzo, Günther K. Bonn, András Guttman

Research output: Contribution to journalArticle

58 Citations (Scopus)

Abstract

Given the high specificity of plant lectins, it is not surprising that their immobilized forms are more and more frequently used for affinity-based analysis of glycoproteins. Common lectin-immobilization techniques range from reversible, non-covalent attachments to covalent immobilization onto a vast palette of substrates (e.g., agarose, silica, and polymeric materials). This article gives an overview of recently reported strategies in lectin immobilization to different stationary phases in chromatography and electrophoresis in order to support lectin-affinity-interaction-based bioseparation methodologies.

Original languageEnglish
Pages (from-to)423-432
Number of pages10
JournalTrAC - Trends in Analytical Chemistry
Volume26
Issue number5
DOIs
Publication statusPublished - May 1 2007

Keywords

  • Boronic acid
  • Glycoprotein
  • Immobilization
  • Lectin affinity
  • Separation

ASJC Scopus subject areas

  • Analytical Chemistry
  • Spectroscopy

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