Leaf hue measurements: A high-throughput screening of chlorophyll content

Research output: Chapter in Book/Report/Conference proceedingChapter

8 Citations (Scopus)


Computer analysis of digital photographic images provides fast, high-throughput screening of leaf pigmentation. Pixel-by-pixel conversion of red, green, blue (RGB) parameters to hue, saturation, value (HSV) showed that Hue values were proportional to total chlorophyll, offering an alternative to photometric analysis of leaf extracts. This is demonstrated using tobacco leaves with various chlorophyll contents due to senescence but shows the possibility of applications in studies of stress conditions accompanied by chlorophyll loss.

Original languageEnglish
Title of host publicationHigh-Throughput Phenotyping in Plants
Subtitle of host publicationMethods and Protocols
PublisherHumana Press Inc.
Number of pages9
ISBN (Print)9781617799945
Publication statusPublished - Jan 1 2012

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745



  • Chlorophyll fluorescence
  • HSV color model
  • High-throughput screening
  • Leaf Hue
  • Photochemical yield
  • RGB color model

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Sass, L., Majer, P., & Hideg, É. (2012). Leaf hue measurements: A high-throughput screening of chlorophyll content. In High-Throughput Phenotyping in Plants: Methods and Protocols (pp. 61-69). (Methods in Molecular Biology; Vol. 918). Humana Press Inc.. https://doi.org/10.1007/978-1-61779-995-2_6