Leaf hue measurements

A high-throughput screening of chlorophyll content

L. Sass, Petra Majer, É. Hideg

Research output: Chapter in Book/Report/Conference proceedingChapter

8 Citations (Scopus)

Abstract

Computer analysis of digital photographic images provides fast, high-throughput screening of leaf pigmentation. Pixel-by-pixel conversion of red, green, blue (RGB) parameters to hue, saturation, value (HSV) showed that Hue values were proportional to total chlorophyll, offering an alternative to photometric analysis of leaf extracts. This is demonstrated using tobacco leaves with various chlorophyll contents due to senescence but shows the possibility of applications in studies of stress conditions accompanied by chlorophyll loss.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages61-69
Number of pages9
Volume918
ISBN (Print)9781617799945
DOIs
Publication statusPublished - 2012

Publication series

NameMethods in Molecular Biology
Volume918
ISSN (Print)10643745

Fingerprint

Chlorophyll
Pigmentation
Tobacco

Keywords

  • Chlorophyll fluorescence
  • High-throughput screening
  • HSV color model
  • Leaf Hue
  • Photochemical yield
  • RGB color model

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Sass, L., Majer, P., & Hideg, É. (2012). Leaf hue measurements: A high-throughput screening of chlorophyll content. In Methods in Molecular Biology (Vol. 918, pp. 61-69). (Methods in Molecular Biology; Vol. 918). Humana Press Inc.. https://doi.org/10.1007/978-1-61779-995-2_6

Leaf hue measurements : A high-throughput screening of chlorophyll content. / Sass, L.; Majer, Petra; Hideg, É.

Methods in Molecular Biology. Vol. 918 Humana Press Inc., 2012. p. 61-69 (Methods in Molecular Biology; Vol. 918).

Research output: Chapter in Book/Report/Conference proceedingChapter

Sass, L, Majer, P & Hideg, É 2012, Leaf hue measurements: A high-throughput screening of chlorophyll content. in Methods in Molecular Biology. vol. 918, Methods in Molecular Biology, vol. 918, Humana Press Inc., pp. 61-69. https://doi.org/10.1007/978-1-61779-995-2_6
Sass L, Majer P, Hideg É. Leaf hue measurements: A high-throughput screening of chlorophyll content. In Methods in Molecular Biology. Vol. 918. Humana Press Inc. 2012. p. 61-69. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-61779-995-2_6
Sass, L. ; Majer, Petra ; Hideg, É. / Leaf hue measurements : A high-throughput screening of chlorophyll content. Methods in Molecular Biology. Vol. 918 Humana Press Inc., 2012. pp. 61-69 (Methods in Molecular Biology).
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