Lead-induced alterations in rat kidneys and testes in vivo

Peter Massanyi, Norbert Lukac, Alexander V. Makarevich, Peter Chrenek, Z. Forgács, Marian Zakrzewski, Robert Stawarz, Robert Toman, Peter Lazor, Slavka Flesarova

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

The purpose of this study was to assess the effects of lead administration on the kidney and testicular structure of adult rats. Rats received lead (PbNO3) in single intraperitoneal dose 50 mg/kg (group A), 25 mg/kg (group B) and 12.5 mg (group C) per kilogram of body weight and were killed 48 h following lead administration. After the preparation of histological samples the results were compared with control. After the lead administration dilated Bowman's capsules and blood vessels in interstitium of kidney with evident hemorrhagic alterations were noted. Quantitative analysis determined increased relative volume of interstitium and tubules. Also, the diameter of renal corpuscules, diameter of glomeruli and diameter of Bowman's capsule were significantly increased, especially in group A, with the highest lead concentration. In testes, dilatation of blood capillaries in interstitium, undulation of basal membrane and occurrence of empty spaces in seminiferous epithelium were detected. An apoptosis assay confirmed increased incidence of apoptosis in the spermatogenetic cells after the lead administration. Also further morphometric analysis showed significant differences in evaluated parameters between control and treated groups. The number of cell nuclei was decreased in lead-treated groups, which is concerned with the occurrence of empty spaces as well as with the higher apoptosis incidence in germinal epithelium. This study reports a negative effect of lead on the structure and function of kidney and testes.

Original languageEnglish
Pages (from-to)671-676
Number of pages6
JournalJournal of Environmental Science and Health - Part A Toxic/Hazardous Substances and Environmental Engineering
Volume42
Issue number5
DOIs
Publication statusPublished - Apr 2007

Fingerprint

Rats
Lead
apoptosis
Cell death
Apoptosis
Capsules
blood
Blood vessels
quantitative analysis
Assays
Blood
vessel
Cells
assay
membrane
Membranes
Chemical analysis

Keywords

  • Kidney
  • Lead
  • Rat
  • Testis
  • Toxic effects

ASJC Scopus subject areas

  • Environmental Engineering
  • Environmental Science(all)
  • Environmental Chemistry

Cite this

Lead-induced alterations in rat kidneys and testes in vivo. / Massanyi, Peter; Lukac, Norbert; Makarevich, Alexander V.; Chrenek, Peter; Forgács, Z.; Zakrzewski, Marian; Stawarz, Robert; Toman, Robert; Lazor, Peter; Flesarova, Slavka.

In: Journal of Environmental Science and Health - Part A Toxic/Hazardous Substances and Environmental Engineering, Vol. 42, No. 5, 04.2007, p. 671-676.

Research output: Contribution to journalArticle

Massanyi, P, Lukac, N, Makarevich, AV, Chrenek, P, Forgács, Z, Zakrzewski, M, Stawarz, R, Toman, R, Lazor, P & Flesarova, S 2007, 'Lead-induced alterations in rat kidneys and testes in vivo', Journal of Environmental Science and Health - Part A Toxic/Hazardous Substances and Environmental Engineering, vol. 42, no. 5, pp. 671-676. https://doi.org/10.1080/10934520701244474
Massanyi, Peter ; Lukac, Norbert ; Makarevich, Alexander V. ; Chrenek, Peter ; Forgács, Z. ; Zakrzewski, Marian ; Stawarz, Robert ; Toman, Robert ; Lazor, Peter ; Flesarova, Slavka. / Lead-induced alterations in rat kidneys and testes in vivo. In: Journal of Environmental Science and Health - Part A Toxic/Hazardous Substances and Environmental Engineering. 2007 ; Vol. 42, No. 5. pp. 671-676.
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