Abstract
An LC-UV screening method for simultaneous determination of ascorbic acid (C), and the free forms of thiamine (B1) riboflavin (B2), niacin (B3), pyridoxine (B6) in enriched food products was developed and validated. The chromatographic separation was accomplished within 18 min using a gradient of water with 0.1% formic acid (pH 2.5) and methanol with 0.1% formic acid on a C18 reverse phase column (5 μm, 150 × 3.2 mm) while detection was performed at two wavelengths (266 and 290 nm). Sample preparation was based on an extraction method originally developed for vitamin C. This procedure besides extracting vitamin C was extended to the extraction of the free forms of vitamins B1, B2, B 3, B6 and B9. The developed analytical method was successfully applied for the simultaneous determination of the vitamin C content along with the free vitamin B forms of three different enriched food products.
| Original language | English |
|---|---|
| Pages (from-to) | 1069-1074 |
| Number of pages | 6 |
| Journal | Chromatographia |
| Volume | 71 |
| Issue number | 11-12 |
| DOIs | |
| Publication status | Published - 2010 |
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Keywords
- Cereal, cacao and fruit juice
- Column liquid chromatography
- Enriched foodstuff
- Water-soluble vitamins
ASJC Scopus subject areas
- Analytical Chemistry
- Biochemistry
- Clinical Biochemistry
- Organic Chemistry
Cite this
LC simultaneous determination of the free forms of B group vitamins and vitamin C in various fortified food products. / Engel, Rita; Stefanovits-Banyai, E.; Abrankó, L.
In: Chromatographia, Vol. 71, No. 11-12, 2010, p. 1069-1074.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - LC simultaneous determination of the free forms of B group vitamins and vitamin C in various fortified food products
AU - Engel, Rita
AU - Stefanovits-Banyai, E.
AU - Abrankó, L.
PY - 2010
Y1 - 2010
N2 - An LC-UV screening method for simultaneous determination of ascorbic acid (C), and the free forms of thiamine (B1) riboflavin (B2), niacin (B3), pyridoxine (B6) in enriched food products was developed and validated. The chromatographic separation was accomplished within 18 min using a gradient of water with 0.1% formic acid (pH 2.5) and methanol with 0.1% formic acid on a C18 reverse phase column (5 μm, 150 × 3.2 mm) while detection was performed at two wavelengths (266 and 290 nm). Sample preparation was based on an extraction method originally developed for vitamin C. This procedure besides extracting vitamin C was extended to the extraction of the free forms of vitamins B1, B2, B 3, B6 and B9. The developed analytical method was successfully applied for the simultaneous determination of the vitamin C content along with the free vitamin B forms of three different enriched food products.
AB - An LC-UV screening method for simultaneous determination of ascorbic acid (C), and the free forms of thiamine (B1) riboflavin (B2), niacin (B3), pyridoxine (B6) in enriched food products was developed and validated. The chromatographic separation was accomplished within 18 min using a gradient of water with 0.1% formic acid (pH 2.5) and methanol with 0.1% formic acid on a C18 reverse phase column (5 μm, 150 × 3.2 mm) while detection was performed at two wavelengths (266 and 290 nm). Sample preparation was based on an extraction method originally developed for vitamin C. This procedure besides extracting vitamin C was extended to the extraction of the free forms of vitamins B1, B2, B 3, B6 and B9. The developed analytical method was successfully applied for the simultaneous determination of the vitamin C content along with the free vitamin B forms of three different enriched food products.
KW - Cereal, cacao and fruit juice
KW - Column liquid chromatography
KW - Enriched foodstuff
KW - Water-soluble vitamins
UR - http://www.scopus.com/inward/record.url?scp=77953022131&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=77953022131&partnerID=8YFLogxK
U2 - 10.1365/s10337-010-1571-4
DO - 10.1365/s10337-010-1571-4
M3 - Article
AN - SCOPUS:77953022131
VL - 71
SP - 1069
EP - 1074
JO - Chromatographia
JF - Chromatographia
SN - 0009-5893
IS - 11-12
ER -