LC simultaneous determination of the free forms of B group vitamins and vitamin C in various fortified food products

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20 Citations (Scopus)

Abstract

An LC-UV screening method for simultaneous determination of ascorbic acid (C), and the free forms of thiamine (B1) riboflavin (B2), niacin (B3), pyridoxine (B6) in enriched food products was developed and validated. The chromatographic separation was accomplished within 18 min using a gradient of water with 0.1% formic acid (pH 2.5) and methanol with 0.1% formic acid on a C18 reverse phase column (5 μm, 150 × 3.2 mm) while detection was performed at two wavelengths (266 and 290 nm). Sample preparation was based on an extraction method originally developed for vitamin C. This procedure besides extracting vitamin C was extended to the extraction of the free forms of vitamins B1, B2, B 3, B6 and B9. The developed analytical method was successfully applied for the simultaneous determination of the vitamin C content along with the free vitamin B forms of three different enriched food products.

Original languageEnglish
Pages (from-to)1069-1074
Number of pages6
JournalChromatographia
Volume71
Issue number11-12
DOIs
Publication statusPublished - 2010

Fingerprint

Fortified Food
Vitamin B Complex
formic acid
Vitamins
Ascorbic Acid
Riboflavin
Thiamine
Pyridoxine
Niacin
Methanol
Screening
Wavelength
Water

Keywords

  • Cereal, cacao and fruit juice
  • Column liquid chromatography
  • Enriched foodstuff
  • Water-soluble vitamins

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry
  • Organic Chemistry

Cite this

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title = "LC simultaneous determination of the free forms of B group vitamins and vitamin C in various fortified food products",
abstract = "An LC-UV screening method for simultaneous determination of ascorbic acid (C), and the free forms of thiamine (B1) riboflavin (B2), niacin (B3), pyridoxine (B6) in enriched food products was developed and validated. The chromatographic separation was accomplished within 18 min using a gradient of water with 0.1{\%} formic acid (pH 2.5) and methanol with 0.1{\%} formic acid on a C18 reverse phase column (5 μm, 150 × 3.2 mm) while detection was performed at two wavelengths (266 and 290 nm). Sample preparation was based on an extraction method originally developed for vitamin C. This procedure besides extracting vitamin C was extended to the extraction of the free forms of vitamins B1, B2, B 3, B6 and B9. The developed analytical method was successfully applied for the simultaneous determination of the vitamin C content along with the free vitamin B forms of three different enriched food products.",
keywords = "Cereal, cacao and fruit juice, Column liquid chromatography, Enriched foodstuff, Water-soluble vitamins",
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T1 - LC simultaneous determination of the free forms of B group vitamins and vitamin C in various fortified food products

AU - Engel, Rita

AU - Stefanovits-Banyai, E.

AU - Abrankó, L.

PY - 2010

Y1 - 2010

N2 - An LC-UV screening method for simultaneous determination of ascorbic acid (C), and the free forms of thiamine (B1) riboflavin (B2), niacin (B3), pyridoxine (B6) in enriched food products was developed and validated. The chromatographic separation was accomplished within 18 min using a gradient of water with 0.1% formic acid (pH 2.5) and methanol with 0.1% formic acid on a C18 reverse phase column (5 μm, 150 × 3.2 mm) while detection was performed at two wavelengths (266 and 290 nm). Sample preparation was based on an extraction method originally developed for vitamin C. This procedure besides extracting vitamin C was extended to the extraction of the free forms of vitamins B1, B2, B 3, B6 and B9. The developed analytical method was successfully applied for the simultaneous determination of the vitamin C content along with the free vitamin B forms of three different enriched food products.

AB - An LC-UV screening method for simultaneous determination of ascorbic acid (C), and the free forms of thiamine (B1) riboflavin (B2), niacin (B3), pyridoxine (B6) in enriched food products was developed and validated. The chromatographic separation was accomplished within 18 min using a gradient of water with 0.1% formic acid (pH 2.5) and methanol with 0.1% formic acid on a C18 reverse phase column (5 μm, 150 × 3.2 mm) while detection was performed at two wavelengths (266 and 290 nm). Sample preparation was based on an extraction method originally developed for vitamin C. This procedure besides extracting vitamin C was extended to the extraction of the free forms of vitamins B1, B2, B 3, B6 and B9. The developed analytical method was successfully applied for the simultaneous determination of the vitamin C content along with the free vitamin B forms of three different enriched food products.

KW - Cereal, cacao and fruit juice

KW - Column liquid chromatography

KW - Enriched foodstuff

KW - Water-soluble vitamins

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