LC enantiomeric separation of unusual amino acids using cyclodextrin-based stationary phases

Jeffrey W. Remsburg, Daniel W. Armstrong, A. Péter, Géza Tóth

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

The use of cyclodextrin based stationary phases was investigated for the enantiomeric separation of 20 unusual amino acids. Mobile phase, pH effects, and flow rate were optimized for each separation. Separations were limited to aqueous mobile phases. Nineteen of the amino acids were separated, with seven having a resolution ≥1.5. The highest selectivities came from the alpha, acetylated beta, and 2,6-dinitrophenyl-4-trifluoromethylphenyl derivitized beta-cyclodextrin stationary phases. Amino acids containing a 1,2,3,4 tetrahydroisoquinoline carboxylic acid structure showed great compatibility with the acetylated beta-cyclodextrin. Tyrosine analogues, due to lack of retention, were not well suited to the cyclodextrin stationary phases.

Original languageEnglish
Pages (from-to)219-230
Number of pages12
JournalJournal of Liquid Chromatography and Related Technologies
Volume31
Issue number2
DOIs
Publication statusPublished - Jan 2008

Fingerprint

Cyclodextrins
Amino Acids
pH effects
Tyrosine
Flow rate
betadex

Keywords

  • Amino acids
  • Cyclodextrin stationary phases
  • Enantiomeric separation
  • HPLC

ASJC Scopus subject areas

  • Analytical Chemistry
  • Clinical Biochemistry

Cite this

LC enantiomeric separation of unusual amino acids using cyclodextrin-based stationary phases. / Remsburg, Jeffrey W.; Armstrong, Daniel W.; Péter, A.; Tóth, Géza.

In: Journal of Liquid Chromatography and Related Technologies, Vol. 31, No. 2, 01.2008, p. 219-230.

Research output: Contribution to journalArticle

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