Layer formations of dipalmitoylphosphatidylcholine liposomes in the pretransition range

A. Bóta, Tamás Drucker, Manfred Kriechbaum, Zsolt Pálfia, Gábor Réz

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Destroyed lamellar and chain packing arrangements of the dipalmitoylphosphocholine-water (30% w/w) system are formed in the pretransition range (31-35.5 °C) under quasi-equilibrium conditions observed by means of simultaneous small- and wide-angle X-ray scattering method. The peak profiles of the scattering curves detected in the pretransition range were modeled as the superpositions of the fitted profiles of the Lβ′ and Pβ′ phases corresponding to the one-dimensional layer arrangements and the subcells in the chain packing. On the basis of the fitted profiles the ratios of the phases can be given as a function of the temperature. It can be stated that (i) both the Lβ′ and Pβ′ phases are present in the temperature range of the pretransition and (ii) in the Pβ′ phase the layer arrangements and chain packing do not change to the same extent. The maximum loss in the layer arrangement was observed around 33 °C. The accumulation of the defect structures appears to be highly permanent as it was observed after sequential heat treatments, i.e., quenching to the temperature domain of the Lβ′ phase (28 °C) and then reheating above the pretransition range to the temperature domain of the Pβ′ phase (38 °C). A memory effect occurs in the sense that the destroyed structures are restored in the temperature range of phase Pβ′ after the formation of a largely reconstructed phase Lβ′. The destroyed layer structures formed in the pretransition and in the rippled phase temperature domains are not identical, as observed by freeze-fracture electron microscopy.

Original languageEnglish
Pages (from-to)3101-3108
Number of pages8
JournalLangmuir
Volume15
Issue number9
DOIs
Publication statusPublished - Apr 27 1999

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1,2-Dipalmitoylphosphatidylcholine
Liposomes
Temperature
temperature
profiles
Defect structures
X ray scattering
scattering
Electron microscopy
Quenching
electron microscopy
heat treatment
quenching
Heat treatment
Scattering
Data storage equipment
heating
Water
defects
curves

ASJC Scopus subject areas

  • Physical and Theoretical Chemistry
  • Colloid and Surface Chemistry

Cite this

Layer formations of dipalmitoylphosphatidylcholine liposomes in the pretransition range. / Bóta, A.; Drucker, Tamás; Kriechbaum, Manfred; Pálfia, Zsolt; Réz, Gábor.

In: Langmuir, Vol. 15, No. 9, 27.04.1999, p. 3101-3108.

Research output: Contribution to journalArticle

Bóta, A, Drucker, T, Kriechbaum, M, Pálfia, Z & Réz, G 1999, 'Layer formations of dipalmitoylphosphatidylcholine liposomes in the pretransition range', Langmuir, vol. 15, no. 9, pp. 3101-3108. https://doi.org/10.1021/la980507b
Bóta, A. ; Drucker, Tamás ; Kriechbaum, Manfred ; Pálfia, Zsolt ; Réz, Gábor. / Layer formations of dipalmitoylphosphatidylcholine liposomes in the pretransition range. In: Langmuir. 1999 ; Vol. 15, No. 9. pp. 3101-3108.
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