Layer chromatography-bioassays directed screening and identification of antibacterial compounds from Scotch thistle

Ágnes M. Móricz, Dániel Krüzselyi, Ágnes Alberti, András Darcsi, Györgyi Horváth, Péter Csontos, Szabolcs Béni, Péter G. Ott

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The antibacterial profiling of Onopordum acanthium L. leaf extract and subsequent targeted identification of active compounds is demonstrated. Thin-layer chromatography (TLC) and off-line overpressured layer chromatography (OPLC) coupled with direct bioautography were utilized for investigation of the extract against eight bacterial strains including two plant and three human pathogens and a soil, a marine and a probiotic human gut bacteria. Antibacterial fractions obtaining infusion-transfusion OPLC were transferred to HPLC–MS/MS analysis that resulted in the characterization of three active compounds and two of them were identified as, linoleic and linolenic acid. OPLC method was adopted to preparative-scale flash chromatography for the isolation of the third active compound, which was identified after a further semi-preparative HPLC purification as the germacranolide sesquiterpene lactone onopordopicrin. Pure onopordopicrin exhibited antibacterial activity that was specified as minimal inhibitory concentration in the liquid phase as well.

Original languageEnglish
Pages (from-to)266-272
Number of pages7
JournalJournal of Chromatography A
Volume1524
DOIs
Publication statusPublished - Nov 17 2017

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Keywords

  • Bioassay-guided isolation
  • Lactobacillus plantarum
  • Overpressured layer chromatography-UV/fractionation
  • Overpressured layer chromatography-direct bioautography
  • Thin-layer chromatography-direct bioautography

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry

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