Layer-by-layer nano-assembly of charged polyelectrolytes for label-free optical biosensing with nanostructured materials: The case of nanostructured porous silicon interferometers

S. Mariani, V. Robbiano, L. M. Strambini, A. Debrassi, G. Egri, L. Dähne, G. Barillaro

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

The nano-assembly of charged polyelectrolytes via layer-by-layer (LbL) technology on porous silicon (PSi) interferometers is here demonstrated as an effective biofunctionalization approach for high-sensitivity/selectivity labelfree optical biosensing, using streptavidin/biotin affinity detection as case study. Nanostructured PSi interferometers are biofunctionalized with a nano-assembly of a positively-charged polyelectrolyte, namely, PAH (poly(allylamine hydrochloride)), and a negatively-charged biotinylated polyelectrolyte, namely, b-PMAA (poly(methacrylic acid)), via LbL technology. The nano-assembly is stable under operating conditions and enables the selective and sensitive detection of streptavidin with a sub-picomolar detection limit (namely, DL=0.6 pM), which is 105-fold lower than that achieved with PSi interferometers biofunctionalized using standard silane chemistry. Remarkably, the analytical performance achieved for LbL-biofunctionalized PSi interferometers is comparable to those of state-of-the-art label-free photonic and plasmonic platforms.

Original languageEnglish
Title of host publicationFrontiers in Biological Detection
Subtitle of host publicationFrom Nanosensors to Systems XI
EditorsAmos Danielli, Benjamin L. Miller, Sharon M. Weiss
PublisherSPIE
ISBN (Electronic)9781510624320
DOIs
Publication statusPublished - Jan 1 2019
EventFrontiers in Biological Detection: From Nanosensors to Systems XI 2019 - San Francisco, United States
Duration: Feb 3 2019Feb 4 2019

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume10895
ISSN (Print)1605-7422

Conference

ConferenceFrontiers in Biological Detection: From Nanosensors to Systems XI 2019
CountryUnited States
CitySan Francisco
Period2/3/192/4/19

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Keywords

  • Affinity biosensing
  • Biotin
  • Interferometer
  • Label-free
  • Layer-by-Layer
  • Nano-assembly
  • Optical biosensing
  • Porous silicon
  • Streptavidin

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics
  • Biomaterials
  • Radiology Nuclear Medicine and imaging

Cite this

Mariani, S., Robbiano, V., Strambini, L. M., Debrassi, A., Egri, G., Dähne, L., & Barillaro, G. (2019). Layer-by-layer nano-assembly of charged polyelectrolytes for label-free optical biosensing with nanostructured materials: The case of nanostructured porous silicon interferometers. In A. Danielli, B. L. Miller, & S. M. Weiss (Eds.), Frontiers in Biological Detection: From Nanosensors to Systems XI [1089503] (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 10895). SPIE. https://doi.org/10.1117/12.2508847