Latent immunization to produce high-affinity monoclonal antibodies to progesterone

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

A simple immunization method to obtain high-affinity monoclonal antibodies to progesterone is described in this article. The method is based on the theory of affinity maturation. A long interval between antigen priming and booster ('latent immunization') permits an undisturbed completion of affinity maturation, resulting in the accumulation of memory B lymphocytes with high- affinity Ig receptors, and consequently, in a higher rate of hybridoma clones producing high-affinity antibody after cell fusion. Antibodies obtained after hyperimmunization and latent immunization are compared in a homologous, direct, competitive ELISA. The average numbers of high-affinity antibodies per fusion were 1.3 and 5.7 in the hyperimmunized and latent immunized groups, respectively. There was no significant difference in the specificities between the two immunization groups.

Original languageEnglish
Pages (from-to)79-84
Number of pages6
JournalHybridoma
Volume14
Issue number1
Publication statusPublished - 1995

Fingerprint

Progesterone
Immunization
Antibody Affinity
Monoclonal Antibodies
Secondary Immunization
Cell Fusion
Hybridomas
B-Lymphocytes
Clone Cells
Enzyme-Linked Immunosorbent Assay
Antigens
Antibodies

ASJC Scopus subject areas

  • Genetics
  • Immunology

Cite this

Latent immunization to produce high-affinity monoclonal antibodies to progesterone. / Siklodi, B.; Barna-Vetro, I.; Solti, L.

In: Hybridoma, Vol. 14, No. 1, 1995, p. 79-84.

Research output: Contribution to journalArticle

@article{8b4153ac2ebd4aa6ab4b9eb7dc797d10,
title = "Latent immunization to produce high-affinity monoclonal antibodies to progesterone",
abstract = "A simple immunization method to obtain high-affinity monoclonal antibodies to progesterone is described in this article. The method is based on the theory of affinity maturation. A long interval between antigen priming and booster ('latent immunization') permits an undisturbed completion of affinity maturation, resulting in the accumulation of memory B lymphocytes with high- affinity Ig receptors, and consequently, in a higher rate of hybridoma clones producing high-affinity antibody after cell fusion. Antibodies obtained after hyperimmunization and latent immunization are compared in a homologous, direct, competitive ELISA. The average numbers of high-affinity antibodies per fusion were 1.3 and 5.7 in the hyperimmunized and latent immunized groups, respectively. There was no significant difference in the specificities between the two immunization groups.",
author = "B. Siklodi and I. Barna-Vetro and L. Solti",
year = "1995",
language = "English",
volume = "14",
pages = "79--84",
journal = "Monoclonal Antibodies in Immunodiagnosis and Immunotherapy",
issn = "2167-9436",
publisher = "Mary Ann Liebert Inc.",
number = "1",

}

TY - JOUR

T1 - Latent immunization to produce high-affinity monoclonal antibodies to progesterone

AU - Siklodi, B.

AU - Barna-Vetro, I.

AU - Solti, L.

PY - 1995

Y1 - 1995

N2 - A simple immunization method to obtain high-affinity monoclonal antibodies to progesterone is described in this article. The method is based on the theory of affinity maturation. A long interval between antigen priming and booster ('latent immunization') permits an undisturbed completion of affinity maturation, resulting in the accumulation of memory B lymphocytes with high- affinity Ig receptors, and consequently, in a higher rate of hybridoma clones producing high-affinity antibody after cell fusion. Antibodies obtained after hyperimmunization and latent immunization are compared in a homologous, direct, competitive ELISA. The average numbers of high-affinity antibodies per fusion were 1.3 and 5.7 in the hyperimmunized and latent immunized groups, respectively. There was no significant difference in the specificities between the two immunization groups.

AB - A simple immunization method to obtain high-affinity monoclonal antibodies to progesterone is described in this article. The method is based on the theory of affinity maturation. A long interval between antigen priming and booster ('latent immunization') permits an undisturbed completion of affinity maturation, resulting in the accumulation of memory B lymphocytes with high- affinity Ig receptors, and consequently, in a higher rate of hybridoma clones producing high-affinity antibody after cell fusion. Antibodies obtained after hyperimmunization and latent immunization are compared in a homologous, direct, competitive ELISA. The average numbers of high-affinity antibodies per fusion were 1.3 and 5.7 in the hyperimmunized and latent immunized groups, respectively. There was no significant difference in the specificities between the two immunization groups.

UR - http://www.scopus.com/inward/record.url?scp=0028914048&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028914048&partnerID=8YFLogxK

M3 - Article

VL - 14

SP - 79

EP - 84

JO - Monoclonal Antibodies in Immunodiagnosis and Immunotherapy

JF - Monoclonal Antibodies in Immunodiagnosis and Immunotherapy

SN - 2167-9436

IS - 1

ER -