Latency type-specific distribution of epigenetic marks at the alternative promoters Cp and Qp of epstein-barr virus

György Fejer, Anita Koroknai, Ferenc Banati, Ildiko Györy, Daniel Salamon, Hans Wolf, Hans Helmut Niller, Janos Minarovits

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Transcripts for the Epstein-Barr virus (EBV)-encoded nuclear antigens are initiated at the alternative promoters Wp, Cp and Qp. Although the host cell-independent activity of Cp is regulated by DNA methylation, Qp is unmethylated independently of its activity. Because histone modifications affect the chromatin structure, we compared the levels of diacetylated histone H3, tetraacetylated histone H4 and histone H3 dimethylated on lysine 4 (H3K4me2) at Cp and Qp, in well characterized cell lines representing the major EBV latency types. We found an activity-dependent histone code: acetylated histones marked active Cp, whereas active Qp was selectively enriched both in acetylated histones and H3K4me2. We concluded that active (but not silent) Cp and Qp are located to 'acetylation islands' in latent, episomal EBV genomes, similar to the active chromatin domains of the human genome.

Original languageEnglish
Pages (from-to)1364-1370
Number of pages7
JournalJournal of General Virology
Volume89
Issue number6
DOIs
Publication statusPublished - Jun 1 2008

ASJC Scopus subject areas

  • Virology

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