Latency is the major determinant of UDP-glucuronosyltransferase activity in isolated hepatocytes

Gábor Bánhegyi, Tamás Garzó, Rosella Fulceri, Angelo Benedetti, József Mandl

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28 Citations (Scopus)

Abstract

The glucuronidation of p-nitrophenol was measured in intact, saponin- and alamethicin-treated isolated mouse hepatocytes. In saponin-permeabilized cells the elevation of extrareticular UDP-glucuronic acid concentration enhanced the rate of glucuronidation threefold. When intracellular membranes were also permeabilized by alamethicin, a further tenfold increase in the glucuronidation of p-nitrophenol was present. Parallel measurements of the ER mannose 6-phosphatase activity revealed that saponin selectively permeabilized the plasma membrane, whereas alamethicin permeabilized both plasma membrane and ER membranes. The inhibition of p-nitrophenol glucuronidation by dbcAMP in intact hepatocytes was still present in saponin-treated cells and disappeared in alamethicin-permeabilized hepatocytes. It is suggested that the permeability of the endoplasmic reticulum membrane is a major determinant of glucuronidation not only in microsomes but in isolated hepatocytes as well.

Original languageEnglish
Pages (from-to)149-152
Number of pages4
JournalFEBS letters
Volume328
Issue number1-2
DOIs
Publication statusPublished - Aug 9 1993

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Keywords

  • Alamethicin
  • Compartmentation
  • Mouse hepatocyte
  • Permeabilized cell
  • Saponin
  • UDP-glucuronosyltransferase

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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