Late expression of FosB transcription factor in 4-aminopyridine-induced seizures in the rat cerebral cortex

Andrea Bagosi, Mónika Bakos, B. Krisztin-Péva, A. Mihály

Research output: Contribution to journalArticle

Abstract

In this study, the immunolocalization of FosB transcription factor was investigated in acute and chronic experimental models of seizures induced by 4-aminopyridine. Wistar rats were injected intraperitoneally daily with 5 mg/kg 4-aminopyridine for 1, 4, 8 and 12 days and sacrificed 24 h after the last injection. Corresponding control groups received the solvent of 4-aminopyridine. Immunohistochemistry revealed an increase in FosB immunolabelling in the frontal cortex in 4-aminopyridine-treated animals compared to controls, both in acute and chronic time course groups. The dentate gyrus displayed elevated FosB immunopositivity only after repeatedly applied convulsant (4-aminopyridine), i.e. following 4, 8 and 12 days of treatment, but no significant immunolocalization was observed in the hippocampus proper. The neuronal localization of FosB after 12 days of 4-aminopyridine-induced convulsions was analysed by means of FosB-parvalbumin double immunolabelling. The increased number of double-labelled cells was significant in the frontal cortex, hilum of the dentate fascia and region CA1 of the hippocampus. We conclude that the studied neocortical and allocortical areas showed a different pattern of FosB immunolocalization, which suggests a relative deficiency of transcriptional regulation in the Ammon's horn and may be responsible for distinct response to seizure-induced cellular insult.

Original languageEnglish
Pages (from-to)418-426
Number of pages9
JournalActa Histochemica
Volume110
Issue number5
DOIs
Publication statusPublished - Sep 8 2008

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4-Aminopyridine
Cerebral Cortex
Seizures
Transcription Factors
Hippocampus
Dentate Gyrus
Frontal Lobe
Convulsants
Parvalbumins
Wistar Rats
Theoretical Models
Immunohistochemistry
Control Groups
Injections

Keywords

  • 4-aminopyridine
  • FosB
  • Hippocampus
  • Immunohistochemistry
  • Parvalbumin
  • Rat
  • Seizure

ASJC Scopus subject areas

  • Cell Biology

Cite this

Late expression of FosB transcription factor in 4-aminopyridine-induced seizures in the rat cerebral cortex. / Bagosi, Andrea; Bakos, Mónika; Krisztin-Péva, B.; Mihály, A.

In: Acta Histochemica, Vol. 110, No. 5, 08.09.2008, p. 418-426.

Research output: Contribution to journalArticle

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