Laser treatment of cultured retinal pigment epithelial cells - Evaluation of the cellular damage in vitro

Antal Szabó, Vince Varga, Tarja Toimela, Kai Hiitelä, Hanna Tähti, Simo S. Oja, I. Süveges, Lotta Salminen

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Purpose: Evaluation of the effects of laser photocoagulation on cultured primary retinal pigment epithelial cells. Methods: Cells were treated by a diode laser (678 nm) with 800 and 1600 mW for 0.186 second. Cell toxicity was tested by the WST-1 assay, and the uptakes of glutamate and γ-aminobutyric acid (GABA) were measured. Results: Laser photocoagulation (1600 mW) caused cell damage and the mitochondrial enzyme activity evaluated by a WST-1 test significantly decreased by 20%-30%. Laser treatment caused a dose-dependent decrease in glutamate uptake but increased GABA uptake. Conclusions: Laser treatment and the laser-induced increase in temperature influence transport processes in retinal pigment epithelial cells and may cause cell damage in the posterior part of the retina.

Original languageEnglish
Pages (from-to)246-255
Number of pages10
JournalJournal of Ocular Pharmacology and Therapeutics
Volume20
Issue number3
DOIs
Publication statusPublished - Jun 2004

Fingerprint

Retinal Pigments
Lasers
Epithelial Cells
Aminobutyrates
Glutamic Acid
Light Coagulation
Therapeutics
Semiconductor Lasers
Retina
In Vitro Techniques
Temperature
Enzymes

ASJC Scopus subject areas

  • Pharmacology (medical)
  • Ophthalmology
  • Pharmacology, Toxicology and Pharmaceutics(all)

Cite this

Laser treatment of cultured retinal pigment epithelial cells - Evaluation of the cellular damage in vitro. / Szabó, Antal; Varga, Vince; Toimela, Tarja; Hiitelä, Kai; Tähti, Hanna; Oja, Simo S.; Süveges, I.; Salminen, Lotta.

In: Journal of Ocular Pharmacology and Therapeutics, Vol. 20, No. 3, 06.2004, p. 246-255.

Research output: Contribution to journalArticle

Szabó, Antal ; Varga, Vince ; Toimela, Tarja ; Hiitelä, Kai ; Tähti, Hanna ; Oja, Simo S. ; Süveges, I. ; Salminen, Lotta. / Laser treatment of cultured retinal pigment epithelial cells - Evaluation of the cellular damage in vitro. In: Journal of Ocular Pharmacology and Therapeutics. 2004 ; Vol. 20, No. 3. pp. 246-255.
@article{35968f2b29b3458aa7eb3daeeff69bf5,
title = "Laser treatment of cultured retinal pigment epithelial cells - Evaluation of the cellular damage in vitro",
abstract = "Purpose: Evaluation of the effects of laser photocoagulation on cultured primary retinal pigment epithelial cells. Methods: Cells were treated by a diode laser (678 nm) with 800 and 1600 mW for 0.186 second. Cell toxicity was tested by the WST-1 assay, and the uptakes of glutamate and γ-aminobutyric acid (GABA) were measured. Results: Laser photocoagulation (1600 mW) caused cell damage and the mitochondrial enzyme activity evaluated by a WST-1 test significantly decreased by 20{\%}-30{\%}. Laser treatment caused a dose-dependent decrease in glutamate uptake but increased GABA uptake. Conclusions: Laser treatment and the laser-induced increase in temperature influence transport processes in retinal pigment epithelial cells and may cause cell damage in the posterior part of the retina.",
author = "Antal Szab{\'o} and Vince Varga and Tarja Toimela and Kai Hiitel{\"a} and Hanna T{\"a}hti and Oja, {Simo S.} and I. S{\"u}veges and Lotta Salminen",
year = "2004",
month = "6",
doi = "10.1089/1080768041223594",
language = "English",
volume = "20",
pages = "246--255",
journal = "Journal of Ocular Pharmacology and Therapeutics",
issn = "1080-7683",
publisher = "Mary Ann Liebert Inc.",
number = "3",

}

TY - JOUR

T1 - Laser treatment of cultured retinal pigment epithelial cells - Evaluation of the cellular damage in vitro

AU - Szabó, Antal

AU - Varga, Vince

AU - Toimela, Tarja

AU - Hiitelä, Kai

AU - Tähti, Hanna

AU - Oja, Simo S.

AU - Süveges, I.

AU - Salminen, Lotta

PY - 2004/6

Y1 - 2004/6

N2 - Purpose: Evaluation of the effects of laser photocoagulation on cultured primary retinal pigment epithelial cells. Methods: Cells were treated by a diode laser (678 nm) with 800 and 1600 mW for 0.186 second. Cell toxicity was tested by the WST-1 assay, and the uptakes of glutamate and γ-aminobutyric acid (GABA) were measured. Results: Laser photocoagulation (1600 mW) caused cell damage and the mitochondrial enzyme activity evaluated by a WST-1 test significantly decreased by 20%-30%. Laser treatment caused a dose-dependent decrease in glutamate uptake but increased GABA uptake. Conclusions: Laser treatment and the laser-induced increase in temperature influence transport processes in retinal pigment epithelial cells and may cause cell damage in the posterior part of the retina.

AB - Purpose: Evaluation of the effects of laser photocoagulation on cultured primary retinal pigment epithelial cells. Methods: Cells were treated by a diode laser (678 nm) with 800 and 1600 mW for 0.186 second. Cell toxicity was tested by the WST-1 assay, and the uptakes of glutamate and γ-aminobutyric acid (GABA) were measured. Results: Laser photocoagulation (1600 mW) caused cell damage and the mitochondrial enzyme activity evaluated by a WST-1 test significantly decreased by 20%-30%. Laser treatment caused a dose-dependent decrease in glutamate uptake but increased GABA uptake. Conclusions: Laser treatment and the laser-induced increase in temperature influence transport processes in retinal pigment epithelial cells and may cause cell damage in the posterior part of the retina.

UR - http://www.scopus.com/inward/record.url?scp=3042769728&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=3042769728&partnerID=8YFLogxK

U2 - 10.1089/1080768041223594

DO - 10.1089/1080768041223594

M3 - Article

C2 - 15279729

AN - SCOPUS:3042769728

VL - 20

SP - 246

EP - 255

JO - Journal of Ocular Pharmacology and Therapeutics

JF - Journal of Ocular Pharmacology and Therapeutics

SN - 1080-7683

IS - 3

ER -