Laser-based techniques for living cell pattern formation

B. Hopp, T. Smausz, Bence Papdi, Z. Bor, András Szabó, Lajos Kolozsvári, Costas Fotakis, Antal Nógrádi

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

In the production of biosensors or artificial tissues a basic step is the immobilization of living cells along the required pattern. In this paper the ability of some promising laser-based methods to influence the interaction between cells and various surfaces is presented. In the first set of experiments laser-induced patterned photochemical modification of polymer foils was used to achieve guided adherence and growth of cells to the modified areas: (a) Polytetrafluoroethylene was irradiated with ArF excimer laser (λ=193 nm, FWHM=20 ns, F=9 mJ/cm2) in presence of triethylene-tetramine liquid photoreagent; (b) a thin carbon layer was produced by KrF excimer laser (λ=248 nm, FWHM=30 ns, F=35 mJ/cm2) irradiation on polyimide surface to influence the cell adherence. It was found that the incorporation of amine groups in the PTFE polymer chain instead of the fluorine atoms can both promote and prevent the adherence of living cells (depending on the applied cell types) on the treated surfaces, while the laser generated carbon layer on polyimide surface did not effectively improve adherence. Our attempts to influence the cell adherence by morphological modifications created by ArF laser irradiation onto polyethylene-terephtalate surface showed a surface-roughness dependence. This method was effective only when the Ra roughness parameter of the developed structure did not exceed the 0.1 micrometer value. Pulsed laser deposition with femtosecond KrF excimer lasers (F=2.2 J/cm2) was effectively used to deposit structured thin films from biomaterials (endothelial cell growth supplement and collagen embedded in starch matrix) to promote the adherence and growth of cells. These results present evidence that some surface can be successfully altered to induce guided cell growth.

Original languageEnglish
Pages (from-to)45-49
Number of pages5
JournalApplied Physics A: Materials Science and Processing
Volume93
Issue number1
DOIs
Publication statusPublished - Oct 2008

Fingerprint

Cells
Lasers
Excimer lasers
excimer lasers
lasers
tetramethylenedisulfotetramine
Polytetrafluoroethylene
Cell growth
Full width at half maximum
cells
Polytetrafluoroethylenes
polyimides
Polyimides
Polymers
Carbon
Surface roughness
irradiation
Fluorine
starches
polytetrafluoroethylene

ASJC Scopus subject areas

  • Materials Science(all)
  • Physics and Astronomy (miscellaneous)

Cite this

Laser-based techniques for living cell pattern formation. / Hopp, B.; Smausz, T.; Papdi, Bence; Bor, Z.; Szabó, András; Kolozsvári, Lajos; Fotakis, Costas; Nógrádi, Antal.

In: Applied Physics A: Materials Science and Processing, Vol. 93, No. 1, 10.2008, p. 45-49.

Research output: Contribution to journalArticle

Hopp, B. ; Smausz, T. ; Papdi, Bence ; Bor, Z. ; Szabó, András ; Kolozsvári, Lajos ; Fotakis, Costas ; Nógrádi, Antal. / Laser-based techniques for living cell pattern formation. In: Applied Physics A: Materials Science and Processing. 2008 ; Vol. 93, No. 1. pp. 45-49.
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