Kutya laryngealis chondrolipomája Esetismertetés

Translated title of the contribution: Laryngeal chondrolipoma in a dog. Case report

Jakab Csaba, Szentgáli Zsolt, Psáder Roland, A. Szász, M. Rusvai, Gálfi Péter, Ifj Horváth László, Horváth Ádám

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Supraglottic pedunculated laryngeal chondrolipoma was diagnosed by laryngoscopy in a ten-year-old male Sarplaninac dog. The exophytic, 3×2 cm, lobulated, grayish-white benign mixed mesenchymal tumour originating from left inside wall of the larynx at the supraglottic area and protruded into the cavity of the larynx with upper respiratory obstruction. The chondrolipoma caused serious mixed type dyspnoe and cyanosis. The benign mesenchymoma was surgically removed. The tumour sample was fixed in 8% neutral buffered (in PBS, pH 7.0) formaldehyde solution for 24 hours at room temperature, dehydrated in a series of ethanol and xylene and embedded in paraffin. The 3-4 μm thick sections were routinely stained with hematoxylin and eosin. Histopathology revealed an unencapsulated tumour composed of multiple islands of hyaline cartilage, interspersed with mature adipose tissue and myxoid tissue. Microscopical features showed no atypia, immature lipoblasts or chondroblasts. There were multifocal areas of chondroid metaplasia. PAS-reaction demonstrated abundant glycogen, mainly in chondrocytes. Tissue of chondrolipoma was examined immunohistochemically. For the primary antibodies, mouse monoclonal antibodies against S-100 protein (1:100; DAKO), vimentin (1:100; DAKO), cytokeratin (1:100; DAKO), desmin (1:50; Novo Castra Laboratories), α-SMA (1:50; Novo Castra Laboratories), claudin-5 (1:100; Zymed) and Ki-67 (1:100; DAKO) were used. Immuno-histochemical staining was performed using the streptavidin-peroxidase procedure: antigen-bound primary antibody was detected using standard avidin-biotin immunoperoxidase complex (DAKO LSAB2 Kit), the chromogen substrate was 3,3 - diamino-benzidine tetrahydrochloride (DAB substrate-chromogen, DAKO, Denmark) in each case. Mayer's hemalaun was used for counter-staining. Immunohistochemically the chondrocytes showed a nucleo-cytoplasmic positivity for S-100 protein, cytoplasmic positivity for vimentin. The lipocytes were negative for S-100 protein, but showed cytoplasmic positivity for vimentin. The chondrolipoma was negative for cytokeratin, desmin, α-SMA and claudin-5 tight junction protein. The proliferation index of chondrolipoma, based on Ki-67 antigen was

Original languageHungarian
Pages (from-to)733-740
Number of pages8
JournalMagyar Allatorvosok Lapja
Volume130
Issue number12
Publication statusPublished - 2008

Fingerprint

S100 Proteins
Vimentin
Claudin-5
Chondrocytes
vimentin
Desmin
Dogs
Larynx
Keratins
case studies
desmin
larynx
dogs
chondrocytes
Mesenchymoma
Claudin-1
Periodic Acid-Schiff Reaction
Ki-67 Antigen
Staining and Labeling
neoplasms

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Csaba, J., Zsolt, S., Roland, P., Szász, A., Rusvai, M., Péter, G., ... Ádám, H. (2008). Kutya laryngealis chondrolipomája Esetismertetés. Magyar Allatorvosok Lapja, 130(12), 733-740.

Kutya laryngealis chondrolipomája Esetismertetés. / Csaba, Jakab; Zsolt, Szentgáli; Roland, Psáder; Szász, A.; Rusvai, M.; Péter, Gálfi; László, Ifj Horváth; Ádám, Horváth.

In: Magyar Allatorvosok Lapja, Vol. 130, No. 12, 2008, p. 733-740.

Research output: Contribution to journalArticle

Csaba, J, Zsolt, S, Roland, P, Szász, A, Rusvai, M, Péter, G, László, IH & Ádám, H 2008, 'Kutya laryngealis chondrolipomája Esetismertetés', Magyar Allatorvosok Lapja, vol. 130, no. 12, pp. 733-740.
Csaba J, Zsolt S, Roland P, Szász A, Rusvai M, Péter G et al. Kutya laryngealis chondrolipomája Esetismertetés. Magyar Allatorvosok Lapja. 2008;130(12):733-740.
Csaba, Jakab ; Zsolt, Szentgáli ; Roland, Psáder ; Szász, A. ; Rusvai, M. ; Péter, Gálfi ; László, Ifj Horváth ; Ádám, Horváth. / Kutya laryngealis chondrolipomája Esetismertetés. In: Magyar Allatorvosok Lapja. 2008 ; Vol. 130, No. 12. pp. 733-740.
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