Lactoferrin during lactation reduces lipopolysaccharide-induced brain injury

Vanessa Ginet, Yohan van de Looij, Volodymyr Petrenko, Audrey Toulotte, J. Kiss, Petra S. Hüppi, Stéphane V. Sizonenko

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12 Citations (Scopus)


Lactoferrin (Lf), component of maternal milk, has antioxidant, anti-inflammatory and antimicrobial properties. Neuroprotective effects of Lf on the immature brain have been recently shown in rodent models of intrauterine growth restriction and cerebral hypoxia/ischemia. Here we postulated that Lf could also have beneficial effects on preterm inflammatory brain injury. Lf was supplemented in maternal food during lactation and lipopolysaccharide (LPS) was injected in subcortical white matter of rat pups at postnatal day 3 (P3). Effect of maternal Lf supplementation was investigated 24 h (P4), 4 (P7), or 21 days (P24) after LPS injection mainly on the striatum. Lateral ventricle and brain structures volumes were quantified. Microstructure was evaluated by diffusion tensor imaging, neurite orientation dispersion and density imaging as well as electron microscopy. Neurochemical profile was measured by 1H-magnetic resonance spectroscopy. GFAP protein, proinflammatory cytokines mRNA expression microglial activation were assessed. Lf displayed neuroprotective effects as shown by reduced LPS-induced ventriculomegaly, brain tissue loss, and microstructural modifications, including myelination deficit. 1H-MRS neurochemical profile was less altered through an antioxidant action of Lf. Despite the lack of effect on LPS-induced proinflammatory cytokines genes expression and on reactive gliosis, microglia was less activated under Lf treatment. In conclusion, Lf supplemented in food during lactation attenuated acute and long-term cerebral LPS-induced alterations. This provides a new evidence for a promising use of Lf as a preventive neuroprotective approach in preterm encephalopathy.

Original languageEnglish
Pages (from-to)323-336
Number of pages14
Issue number3
Publication statusPublished - May 1 2016


  • electron microscopy
  • lactoferrin
  • lipopolysaccharide
  • magnetic resonance spectroscopy and imaging
  • perinatal brain

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry
  • Molecular Medicine

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    Ginet, V., van de Looij, Y., Petrenko, V., Toulotte, A., Kiss, J., Hüppi, P. S., & Sizonenko, S. V. (2016). Lactoferrin during lactation reduces lipopolysaccharide-induced brain injury. BioFactors, 42(3), 323-336.