Spectropolarimetry was used to quantify the guanidinium-lactate and lactate-lactate equilibrium reactions. Association constants for the guanidinium-lactate and lactate-lactate formations are 6.11 and 1.12, respectively, in aqueous solution. The value 6.11 is certainly high among electrostatic interactions in water. This stability, however, can not account for the extremely strong lactate-protein binding, observed by NMR spectroscopy. The molar rotation coefficients for both the hetero-association and homo-association complexes are also calculated. The homo-associative lactate-lactate binding is the first such interaction, whose constant has been determined by spectropolarimetry in aqueous solution.
|Number of pages||4|
|Journal||Acta pharmaceutica Hungarica|
|Publication status||Published - Jan 1 1995|
ASJC Scopus subject areas
- Pharmaceutical Science