Lacosamide diminishes dryness-induced hyperexcitability of corneal cold sensitive nerve terminals

I. Kovács, Lóránt Dienes, Kristóf Perényi, Susana Quirce, Carolina Luna, Kamila Mizerska, M. Carmen Acosta, Carlos Belmonte, Juana Gallar

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Lacosamide is an anti-epileptic drug that is also used for the treatment of painful diabetic neuropathy acting through voltage-gated sodium channels. The aim of this work was to evaluate the effects of acute application of lacosamide on the electrical activity of corneal cold nerve terminals in lacrimo-deficient guinea pigs.Four weeks after unilateral surgical removal of the main lachrimal gland in guinea pigs, corneas were excised and superfused in vitro at 34. °C for extracellular electrophysiological recording of nerve terminal impulse activity of cold thermosensitive nerve terminals. The characteristics of the spontaneous and the stimulus-evoked (cooling ramps from 34. °C to 15. °C) activity before and in presence of lacosamide 100. μM and lidocaine 100. μM were compared.Cold nerve terminals (n=34) recorded from dry eye corneas showed significantly enhanced spontaneous activity (8.0±1.1 vs. 5.2±0.7. imp/s; P0.05) to the irrigation fluid.In summary, the application of lacosamide results in a significant decrease of the augmented spontaneous activity and responsiveness to cold of corneal sensory nerves from tear-deficient animals. Based on these promising results we speculate that lacosamide might be used to reduce the hyperexcitability of corneal cold receptors caused by prolonged ocular surface dryness due to hyposecretory or evaporative dry eye disease.

Original languageEnglish
JournalEuropean Journal of Pharmacology
DOIs
Publication statusAccepted/In press - Mar 30 2016

Fingerprint

Cornea
Guinea Pigs
Voltage-Gated Sodium Channels
Architectural Accessibility
Eye Diseases
Diabetic Neuropathies
Lidocaine
Tears
Action Potentials
lacosamide
Pharmaceutical Preparations
In Vitro Techniques

Keywords

  • Cold thermoreceptors
  • Dry eye
  • Hyperexcitability
  • Lacosamide
  • Sodium channels

ASJC Scopus subject areas

  • Pharmacology

Cite this

Lacosamide diminishes dryness-induced hyperexcitability of corneal cold sensitive nerve terminals. / Kovács, I.; Dienes, Lóránt; Perényi, Kristóf; Quirce, Susana; Luna, Carolina; Mizerska, Kamila; Acosta, M. Carmen; Belmonte, Carlos; Gallar, Juana.

In: European Journal of Pharmacology, 30.03.2016.

Research output: Contribution to journalArticle

Kovács, I. ; Dienes, Lóránt ; Perényi, Kristóf ; Quirce, Susana ; Luna, Carolina ; Mizerska, Kamila ; Acosta, M. Carmen ; Belmonte, Carlos ; Gallar, Juana. / Lacosamide diminishes dryness-induced hyperexcitability of corneal cold sensitive nerve terminals. In: European Journal of Pharmacology. 2016.
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AU - Luna, Carolina

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