Lack of involvement of [Ca2+]i in the external Ca2+-independent release of acetylcholine evoked by veratridine, ouabain and α-latrotoxin: Possible role of [Na+]i

V. Adam-Vizi, Z. Deri, P. Bors, L. Tretter

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Synaptosomes were challenged by veratridine, ouabain and α-latrotoxin, and the release of 14C-acetycholine (ACh) was measured in the absence of external Ca2+. We wished to test whether Ca2+ mobilized from internal stores triggered the ACh release that was independent of external Ca2+. We found that none of the agents altered the [Ca2+i in a Ca2+-free medium. Buffering the intracellular Ca2+ concentration with BAPTA did not prevent the increase in release of 14C-ACh by veratridien or ouabain in the abscence of Ca2+, however, it greatly reduced the released evoked in Ca2+-containing medium. In parallel samples teh release of ACh and the change in the internal Na+ concentration ([Na+]i) were measured. It was found that veratridine, oubain and α-latrotoxin all enhance [Na+]i in a concentration-dependent manner and a good quantitative relationship existed between the increase in [Na+]i and the release of ACh.

Original languageEnglish
Pages (from-to)43-50
Number of pages8
JournalJournal of Physiology - Paris
Volume87
Issue number1
DOIs
Publication statusPublished - 1993

Keywords

  • ACh release
  • external Ca
  • ouabain
  • veratridine
  • α-latrotoxin

ASJC Scopus subject areas

  • Neuroscience(all)
  • Physiology (medical)

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