Lack of involvement of [Ca2+]i in the external Ca2+-independent release of acetylcholine evoked by veratridine, ouabain and α-latrotoxin: Possible role of [Na+]i

V. Ádám-Vizi, Z. Deri, P. Bors, L. Tretter

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Synaptosomes were challenged by veratridine, ouabain and α-latrotoxin, and the release of 14C-acetycholine (ACh) was measured in the absence of external Ca2+. We wished to test whether Ca2+ mobilized from internal stores triggered the ACh release that was independent of external Ca2+. We found that none of the agents altered the [Ca2+i in a Ca2+-free medium. Buffering the intracellular Ca2+ concentration with BAPTA did not prevent the increase in release of 14C-ACh by veratridien or ouabain in the abscence of Ca2+, however, it greatly reduced the released evoked in Ca2+-containing medium. In parallel samples teh release of ACh and the change in the internal Na+ concentration ([Na+]i) were measured. It was found that veratridine, oubain and α-latrotoxin all enhance [Na+]i in a concentration-dependent manner and a good quantitative relationship existed between the increase in [Na+]i and the release of ACh.

Original languageEnglish
Pages (from-to)43-50
Number of pages8
JournalJournal of Physiology Paris
Volume87
Issue number1
DOIs
Publication statusPublished - 1993

Fingerprint

Veratridine
Ouabain
Acetylcholine
Synaptosomes
1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid

Keywords

  • ACh release
  • external Ca
  • ouabain
  • veratridine
  • α-latrotoxin

ASJC Scopus subject areas

  • Physiology (medical)
  • Neuroscience(all)

Cite this

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abstract = "Synaptosomes were challenged by veratridine, ouabain and α-latrotoxin, and the release of 14C-acetycholine (ACh) was measured in the absence of external Ca2+. We wished to test whether Ca2+ mobilized from internal stores triggered the ACh release that was independent of external Ca2+. We found that none of the agents altered the [Ca2+i in a Ca2+-free medium. Buffering the intracellular Ca2+ concentration with BAPTA did not prevent the increase in release of 14C-ACh by veratridien or ouabain in the abscence of Ca2+, however, it greatly reduced the released evoked in Ca2+-containing medium. In parallel samples teh release of ACh and the change in the internal Na+ concentration ([Na+]i) were measured. It was found that veratridine, oubain and α-latrotoxin all enhance [Na+]i in a concentration-dependent manner and a good quantitative relationship existed between the increase in [Na+]i and the release of ACh.",
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author = "V. {\'A}d{\'a}m-Vizi and Z. Deri and P. Bors and L. Tretter",
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AU - Ádám-Vizi, V.

AU - Deri, Z.

AU - Bors, P.

AU - Tretter, L.

PY - 1993

Y1 - 1993

N2 - Synaptosomes were challenged by veratridine, ouabain and α-latrotoxin, and the release of 14C-acetycholine (ACh) was measured in the absence of external Ca2+. We wished to test whether Ca2+ mobilized from internal stores triggered the ACh release that was independent of external Ca2+. We found that none of the agents altered the [Ca2+i in a Ca2+-free medium. Buffering the intracellular Ca2+ concentration with BAPTA did not prevent the increase in release of 14C-ACh by veratridien or ouabain in the abscence of Ca2+, however, it greatly reduced the released evoked in Ca2+-containing medium. In parallel samples teh release of ACh and the change in the internal Na+ concentration ([Na+]i) were measured. It was found that veratridine, oubain and α-latrotoxin all enhance [Na+]i in a concentration-dependent manner and a good quantitative relationship existed between the increase in [Na+]i and the release of ACh.

AB - Synaptosomes were challenged by veratridine, ouabain and α-latrotoxin, and the release of 14C-acetycholine (ACh) was measured in the absence of external Ca2+. We wished to test whether Ca2+ mobilized from internal stores triggered the ACh release that was independent of external Ca2+. We found that none of the agents altered the [Ca2+i in a Ca2+-free medium. Buffering the intracellular Ca2+ concentration with BAPTA did not prevent the increase in release of 14C-ACh by veratridien or ouabain in the abscence of Ca2+, however, it greatly reduced the released evoked in Ca2+-containing medium. In parallel samples teh release of ACh and the change in the internal Na+ concentration ([Na+]i) were measured. It was found that veratridine, oubain and α-latrotoxin all enhance [Na+]i in a concentration-dependent manner and a good quantitative relationship existed between the increase in [Na+]i and the release of ACh.

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KW - α-latrotoxin

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