Lack of correlation between the 257C-to-T mutation in the gyrA gene and clinical severity of Campylobacter jejuni infection in a region of high incidence of ciprofloxacin resistance

Agnes Sonnevend, Judit Kovacs, Tibor Pal, Nadia Akawi, Nico Nagelkerke, G. Schneider

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Background: Fluoroquinolone resistance is increasingly detected in Campylobacter jejuni worldwide. Despite the fact that a point mutation in the gyrA gene has been linked with increased fitness in animals, the association of resistant organisms with more severe infections in man remains controversial. Methods: Erythromycin and quinolone susceptibility of 147 C. jejuni strains isolated from individual patients with diarrhoea in southwest Hungary were investigated and the molecular background of fluoroquinolone resistance was determined. Hospitalization and the presence of macroscopic blood in the stool were correlated with the presence of 257C-to-T mutation of the gyrA gene causing resistance to fluoroquinolones. Results: Isolates showed an extensive genetic heterogeneity by macrorestriction analysis of the chromosome. While all strains retained susceptibility to erythromycin, 68% were non-susceptible to ciprofloxacin. The mutation causing a Thr-86-to-Ile replacement in the gyrA gene was present in 98% of non-susceptible isolates. Infection caused by isolates containing this mutation did not show any significant association with either hospitalization or with the development of bloody diarrhoea. Conclusions: Our findings indicate that in a region with high-level ciprofloxacin resistance in C. jejuni, non-susceptibility to this antibiotic did not correlate with the severity of infection.

Original languageEnglish
Pages (from-to)905-911
Number of pages7
JournalScandinavian Journal of Infectious Diseases
Volume43
Issue number11-12
DOIs
Publication statusPublished - Dec 2011

Fingerprint

Campylobacter Infections
Campylobacter jejuni
Fluoroquinolones
Ciprofloxacin
Erythromycin
Mutation
Diarrhea
Incidence
Hospitalization
Infection
Genes
Genetic Heterogeneity
Hungary
Quinolones
Point Mutation
Chromosomes
Anti-Bacterial Agents

Keywords

  • Campylobacter jejuni
  • Ciprofloxacin resistance
  • Fitness

ASJC Scopus subject areas

  • Microbiology (medical)
  • Immunology and Microbiology(all)
  • Infectious Diseases

Cite this

Lack of correlation between the 257C-to-T mutation in the gyrA gene and clinical severity of Campylobacter jejuni infection in a region of high incidence of ciprofloxacin resistance. / Sonnevend, Agnes; Kovacs, Judit; Pal, Tibor; Akawi, Nadia; Nagelkerke, Nico; Schneider, G.

In: Scandinavian Journal of Infectious Diseases, Vol. 43, No. 11-12, 12.2011, p. 905-911.

Research output: Contribution to journalArticle

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abstract = "Background: Fluoroquinolone resistance is increasingly detected in Campylobacter jejuni worldwide. Despite the fact that a point mutation in the gyrA gene has been linked with increased fitness in animals, the association of resistant organisms with more severe infections in man remains controversial. Methods: Erythromycin and quinolone susceptibility of 147 C. jejuni strains isolated from individual patients with diarrhoea in southwest Hungary were investigated and the molecular background of fluoroquinolone resistance was determined. Hospitalization and the presence of macroscopic blood in the stool were correlated with the presence of 257C-to-T mutation of the gyrA gene causing resistance to fluoroquinolones. Results: Isolates showed an extensive genetic heterogeneity by macrorestriction analysis of the chromosome. While all strains retained susceptibility to erythromycin, 68{\%} were non-susceptible to ciprofloxacin. The mutation causing a Thr-86-to-Ile replacement in the gyrA gene was present in 98{\%} of non-susceptible isolates. Infection caused by isolates containing this mutation did not show any significant association with either hospitalization or with the development of bloody diarrhoea. Conclusions: Our findings indicate that in a region with high-level ciprofloxacin resistance in C. jejuni, non-susceptibility to this antibiotic did not correlate with the severity of infection.",
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