Lack of ATP-evoked GABA and glutamate release in the hippocampus of P2X7 receptor-/- mice

Lilla Papp, E. Vízi, B. Sperlágh

Research output: Contribution to journalArticle

69 Citations (Scopus)

Abstract

In this study we revealed the participation of P2X7 receptors in the modulation of electrical stimulation and ATP-evoked GABA and glutamate release from mouse hippocampal slices. Whereas the uptake of radioactivity was not changed, the electrical stimulation-induced release of both [ 3H]glutamate and [3H]GABA was decreased in the hippocampus of P2X7 receptor-deficient mice. ATP (10 mM) elicited [ 3H]glutamate and [3H]GABA efflux in wildtype mice, which was inhibited by the non-selective P2 receptor antagonist pyridoxal-phosphate-6- azophenyl-2′,4′-disulphonic acid tetrasodium (30 μM) and the P2X7-selective antagonist Brilliant Blue G (1 μM). The ATP-evoked release of both GABA and glutamate was virtually absent in the knockout mice. These results indicate that ATP facilitates GABA and glutamate release in the hippocampus by a mechanism involving P2X7R.

Original languageEnglish
Pages (from-to)2387-2391
Number of pages5
JournalNeuroReport
Volume15
Issue number15
DOIs
Publication statusPublished - Oct 25 2004

Fingerprint

Purinergic P2X7 Receptors
gamma-Aminobutyric Acid
Glutamic Acid
Hippocampus
Adenosine Triphosphate
Electric Stimulation
Knockout Mice
Radioactivity
Acids

Keywords

  • GABA
  • Glutamate
  • Hippocampus
  • Knockout
  • P2X receptor
  • Release

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Lack of ATP-evoked GABA and glutamate release in the hippocampus of P2X7 receptor-/- mice. / Papp, Lilla; Vízi, E.; Sperlágh, B.

In: NeuroReport, Vol. 15, No. 15, 25.10.2004, p. 2387-2391.

Research output: Contribution to journalArticle

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