Labeling phosphorylated LHCII with microspheres for tracking studies and force measurements

Laura Finzi, P. Galajda, G. Garab

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

We report a method to selectively label phosphorylated, membrane proteins with microscopic particles. This technology is particularly useful in single particle studies. In such studies, the particles may serve to visualize protein diffusion and/or as 'handles' to study the force of interaction between the labeled protein and the membrane matrix. In the latter kind of experiments, forces can be applied and measured by calibrated optical tweezers. Optical tweezers were used in this work to test the strength of the particle labeling. Labeling a single protein with a particle produces a long-lived, distinct tag and is particularly useful for proteins in photosynthetic membranes, which contain endogenous fluorophores that would render single flourescent proteins difficult to detect.

Original languageEnglish
Pages (from-to)1-4
Number of pages4
JournalJournal of Photochemistry and Photobiology, B: Biology
Volume65
Issue number1
DOIs
Publication statusPublished - Dec 1 2002

Fingerprint

Force measurement
Microspheres
Labeling
marking
Optical Tweezers
proteins
Proteins
Optical tweezers
Photosynthetic membranes
Membrane Proteins
membranes
Fluorophores
fluorescent dyes
Membranes
Labels
strength (mechanics)
membrane proteins
Technology
Experiments
matrices

Keywords

  • Diffusion
  • LHCII
  • Optical tweezers
  • Protein phosphorylation
  • Single particle tracking

ASJC Scopus subject areas

  • Plant Science
  • Bioengineering
  • Physical and Theoretical Chemistry

Cite this

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AU - Garab, G.

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AB - We report a method to selectively label phosphorylated, membrane proteins with microscopic particles. This technology is particularly useful in single particle studies. In such studies, the particles may serve to visualize protein diffusion and/or as 'handles' to study the force of interaction between the labeled protein and the membrane matrix. In the latter kind of experiments, forces can be applied and measured by calibrated optical tweezers. Optical tweezers were used in this work to test the strength of the particle labeling. Labeling a single protein with a particle produces a long-lived, distinct tag and is particularly useful for proteins in photosynthetic membranes, which contain endogenous fluorophores that would render single flourescent proteins difficult to detect.

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KW - Single particle tracking

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