Label-free optical detection of mycotoxins using specific aptamers immobilized on gold nanostructures

Ali Ghanim Al-Rubaye, Alexei Nabok, Gaelle Catanante, Jean Louis Marty, Eszter Takács, András Székács

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

This work focuses on the development of the novel label-free optical apta-sensors for detection of mycotoxins. A highly sensitive analytical method of total internal reflection ellipsometry (TIRE) combined with Localized Surface Plasmon Resonance (LSPR) phenomenon in nano-structured gold films was exploited here for the first time for detection of aflatoxin B1 and M1 in direct assay with specific aptamers immobilized on the surface of gold. The achieved detection of low molecular weight molecules, such as aflatoxin B1 and M1, in a wide range of concentrations from 100 ng/mL down to 0.01 ng/mL is remarkable for the LSPR method. The study of binding kinetics of aflatoxin molecules to their respective aptamers using dynamic TIRE measurements yielded the values of affinity constants in the range of 10 - 8 –10 - 7 mol, which is characteristic for highly specific aptamer/target interactions similar to that for monoclonal antibodies. The effect of aptamers’ DNA chain length on their binding characteristics was analyzed.

Original languageEnglish
Article number291
JournalToxins
Volume10
Issue number7
DOIs
Publication statusPublished - Jul 16 2018

Keywords

  • Aptamer
  • Gold nanostructures
  • LSPR
  • Mycotoxins
  • Optical label-free biosensor
  • Total internal reflection ellipsometery

ASJC Scopus subject areas

  • Toxicology
  • Health, Toxicology and Mutagenesis

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