Label-free Microfluidic Sensing by Detection of Interaction-triggered Change in Blood Flow Characteristics

Éva Sautner, Krisztián Papp, Eszter Holczer, Rita Ungai-Salánki, B. Szabó, Péter Fürjes, József Prechl

Research output: Contribution to journalArticle

Abstract

Microfluidic devices exploit combined physical, chemical and biological phenomena that could be unique in the sub-millimeter dimensions. The blood is a non-Newtonian fluid, containing particulate and soluble elements, which penetrates the whole body carrying a wealth of biomedical information. The design of microfluidic devices capable of extracting immediately this information is the current goal of development Point-of-Care (POC) medical devices. We examined the characteristics of blood flow in specially designed microfluidic devices having different geometric structure and material composition with the aim of defining suitable conditions for sensitive detection of changes in the interactions between particulate elements of the blood and the adequately modified surfaces of the microfluidic system. As a model experiment we demonstrated the fast analysis of the AB0 blood group system, applying respective antibody reagents and capillary blood samples with different blood groups. We showed that by tuning the hydrophilicity of the surface and capillary dimensions of the microfluidic system it is possible to detect precisely the red blood cell binding to the capillary walls by monitoring the flow rate characteristics in an autonomous microfluidic system. Our proof-of-concept results point to a novel direction in blood analysis in autonomous microfluidic systems and also provide the basis for the construction of a simple quantitative device for blood group determination.

Original languageEnglish
Pages (from-to)1406-1409
Number of pages4
JournalProcedia Engineering
Volume168
DOIs
Publication statusPublished - 2016

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Microfluidics
Labels
Blood
Hydrophilicity
Health care
Antibodies
Tuning
Cells
Flow rate
Fluids
Monitoring
Chemical analysis

Keywords

  • antibody
  • autonomous
  • blood
  • blood group
  • lab-on-a-chip
  • label-free
  • microfluidics
  • sensor

ASJC Scopus subject areas

  • Engineering(all)

Cite this

Label-free Microfluidic Sensing by Detection of Interaction-triggered Change in Blood Flow Characteristics. / Sautner, Éva; Papp, Krisztián; Holczer, Eszter; Ungai-Salánki, Rita; Szabó, B.; Fürjes, Péter; Prechl, József.

In: Procedia Engineering, Vol. 168, 2016, p. 1406-1409.

Research output: Contribution to journalArticle

Sautner, Éva ; Papp, Krisztián ; Holczer, Eszter ; Ungai-Salánki, Rita ; Szabó, B. ; Fürjes, Péter ; Prechl, József. / Label-free Microfluidic Sensing by Detection of Interaction-triggered Change in Blood Flow Characteristics. In: Procedia Engineering. 2016 ; Vol. 168. pp. 1406-1409.
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