L-arginine: Effect on reperfusion injury after heart transplantation

Gábor Szabó, Susanne Bährle, Sándor Bátkai, Nicole Stumpf, Thomas J. Dengler, Rainer Zimmermann, Christian F. Vahl, Siegfried Hagl

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Abstract

Global myocardial ischemia and reperfusion injury play a major role in early postoperative myocardial graft dysfunction. The aim of the present study was to investigate the effects of the nitric oxide (NO) precursor L- arginine on myocardial and endothelial function after hypothermic ischemia and reperfusion in a heterotopic rat heart transplantation model. After 1 hour ischemic preservation, reperfusion was started after application of placebo (control, n = 12) or L-arginine (L-Arg 40 mg/kg, n = 12), a substrate of NO synthesis. Myocardial blood flow (MBF) was assessed by the hydrogen clearance method. An implanted balloon was used to obtain pressure-volume relations of the transplanted heart. Left ventricular developed pressure (LVDP), rate of pressure development (dP/dt), end-diastolic pressure (LVEDP), isovolumic relaxation constant (T(E)), and MBF were measured after 60 minutes and 24 hours of reperfusion. Endothelium-dependent vasodilatation in response to acetylcholine (ACh) and endothelium-independent vasodilatation in response to sodium nitroprusside (SNP) were also determined. After 1 hour the MBF was significantly higher in the L-Arg group (3.6 ± 0.6 vs. 1.9 ± 0.2 ml/min/g, p < 0.05). The L-Arg group showed better recovery of systolic function and myocardial relaxation (LVDP 106 ± 6 vs. 70 ± 7 mmHg, p < 0.05; maximal dP/dt 5145 ± 498 vs. 3410 ± 257 mmHg/s, p < 0.05; T(E) 12.1 ± 0.9 vs. 16.1 ± 1.5 ms, p < 0.05, at an intraventricular volume of 80 μl). LVEDP was similar in the two groups. After 24 hours no difference was found between the groups for basal MBF, LVP, dP/dt, T(E), LVEDP, or the response of MBF to SNP. However, ACh led to a significantly higher increase in MBF in the L-Arg group (52 ± 8% vs. 29 ± 7%, p < 0.05). These results indicate that (1) NO donation improves myocardial and endothelial functional recovery during early reperfusion after heart transplantation; and (2) initial treatment with L- Arg has a persisting beneficial effect against reperfusion-induced graft coronary endothelial dysfunction during late reperfusion.

Original languageEnglish
Pages (from-to)791-798
Number of pages8
JournalWorld journal of surgery
Volume22
Issue number8
DOIs
Publication statusPublished - Aug 1 1998

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ASJC Scopus subject areas

  • Surgery

Cite this

Szabó, G., Bährle, S., Bátkai, S., Stumpf, N., Dengler, T. J., Zimmermann, R., Vahl, C. F., & Hagl, S. (1998). L-arginine: Effect on reperfusion injury after heart transplantation. World journal of surgery, 22(8), 791-798. https://doi.org/10.1007/s002689900471