L-Arabinose induces D-galactose catabolism via the Leloir pathway in Aspergillus nidulans

Zoltán Németh, László Kulcsár, Michel Flipphi, Anita Orosz, Maria Victoria Aguilar-Pontes, Ronald P. de Vries, Levente Karaffa, Erzsébet Fekete

Research output: Contribution to journalArticle

Abstract

L-Arabinose and D-galactose are the principal constituents of L-arabinogalactan, and also co-occur in other hemicelluloses and pectins. In this work we hypothesized that similar to the induction of relevant glycoside hydrolases by monomers liberated from these plant heteropolymers, their respective catabolisms in saprophytic and phytopathogenic fungi may respond to the presence of the other sugar to promote synergistic use of the complex growth substrate. We showed that these two sugars are indeed consumed simultaneously by Aspergillus nidulans, while L-arabinose is utilised faster in the presence than in the absence of D-galactose. Furthermore, the first two genes of the Leloir pathway for D-galactose catabolism – encoding D-galactose 1-epimerase and galactokinase – are induced more rapidly by L-arabinose than by D-galactose eventhough deletion mutants thereof grow as well as a wild type strain on the pentose. D-Galactose 1-epimerase is hyperinduced by L-arabinose, D-xylose and L-arabitol but not by xylitol. The results suggest that in A. nidulans, L-arabinose and D-xylose – both requiring NADPH for their catabolisation – actively promote the enzyme infrastructure necessary to convert β-D-galactopyranose via the Leloir pathway with its α-anomer specific enzymes, into β-D-glucose-6-phosphate (the starting substrate of the oxidative part of the pentose phosphate pathway) even in the absence of D-galactose.

Original languageEnglish
Pages (from-to)53-59
Number of pages7
JournalFungal Genetics and Biology
Volume123
DOIs
Publication statusPublished - Feb 2019

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Keywords

  • D-Galactose
  • Galactokinase gene
  • Galactose 1-epimerase gene
  • Induction of transcription
  • L-Arabinose
  • Mixed carbon source synergism

ASJC Scopus subject areas

  • Microbiology
  • Genetics

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